@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001650, author = "Lin, Jonathan Y. and Hobson, William J. and Wertz, John T.", title = "Saccharedens versatilis gen. nov., sp. nov., a sugar-degrading member of the Burkholderiales isolated from Cephalotes rohweri ant guts", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "2", pages = "447-453", doi = "https://doi.org/10.1099/ijsem.0.001650", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001650", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Saccharedens versatilis", keywords = "gut microbiota", keywords = "Burkholderiales", keywords = "symbiont", keywords = "short-chain fatty acid", keywords = "Cephalotes rohweri", abstract = " Cephalotes ‘turtle’ ants host a core group of gut–associated symbionts, but their potential contributions to ant nutrition and disease resistance remain uncharacterized in vitro. To gain a better understanding of the metabolic capability of core symbionts belonging to the Burkholderiales , we cultivated and characterized strain CAG32T from the guts of Cephalotes rohweri ants. Strain CAG32T was rod-shaped, Gram-stain-negative, motile and formed pale-white colonies on trypticase soy agar. Optimum growth occurred under an atmosphere of 20 % O2 supplemented with 1 % CO2. Strain CAG32T grew under NaCl concentrations of 0–2.0 %, temperatures of 23–47 °C and pH values of 4.0–8.0, and was capable of producing n-butyric acid and degrading carbohydrates for growth. The G+C content of the genomic DNA was 59.2±0.6 mol% and the major fatty acids were C16 : 0, C16 : 1 ω7c/C16  : 1 ω6c, C17 : 0 cylcopropane, C12 : 0 and C14 : 0 3-OH/C16 : 1 iso I. The only respiratory quinone detected was ubiquinone-8 (Q-8) and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on phylogenetic analysis of the 16S rRNA gene sequence, strain CAG32T shared 96.9 % nucleotide similarity with its closest cultivated neighbours Bordetella petrii Se-1111RT and Bordetella bronchiseptica ATCC 19395T. This, combined with differences in the phenotypic and biochemical profile from neighbouring strains, warrants the classification of strain CAG32T as representing a novel species of a new genus within the Burkholderiales family Alcaligenaceae . The name Saccharedens versatilis gen. nov., sp. nov. is proposed. The type strain of Saccharedens versatilis is CAG32T (=NCIMB 15010T=DSM 100909T).", }