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Volume 67, Issue 3

sp. nov., isolated from a maize leaf Free

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, flexirubin-producing, rod-shaped and endophytic bacterium, designated strain CC-YTH209, was isolated from a maize leaf and subjected to a taxonomic study. Strain CC-YTH209 was found to grow at 15–40 °C (optimal 30 °C), at pH 6.0–8.0 (optimal pH 7.0) and in the presence of 0–2 % (optimal 1 %) (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain CC-YTH209 appeared to belong to the genus within the class , and was closely related to CJ16 (97.5 % similarity) and PHA3-4 (96.9 %). The level of DNA–DNA relatedness between strain CC-YTH209 and CJ16 was 14.4 % (reciprocal, 13.0 %). Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-YTH209 within the clade that accommodated species. The DNA G+C content was 37.2 mol%. Strain CC-YTH209 contained menaquinone MK-6 as the predominant respiratory quinone and -homospermidine as the major polyamine. The major cellular fatty acids of the isolate were iso-C, iso-C 3-OH and C 6/C 7. The polar lipid profile comprised phosphatidylethanolamine and five aminolipids, three lipids, one glycolipid, one aminophospholipid and one phospholipid, which are presently uncharacterized. On the basis of evidence presented in this study, strain CC-YTH209 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CC-YTH209 (=BCRC 80938=JCM 31226).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Chryseobacterium endophyticum , Flexirubin and strain CC-YTH209T
© 2017 IUMS
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2017-03-01
2024-04-19
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Chryseobacterium endophyticum sp. nov., isolated from a maize leaf
Int J Syst Evol Microbiol 67, 570 (2017); https://doi.org/10.1099/ijsem.0.001656
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