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Volume 67, Issue 3

gen. nov., sp. nov., a new member of the family () isolated from activated sludge Free

Abstract

A new betaproteobacterium, CGII-59m2, was isolated from an activated sludge bioreactor which treated landfill leachate. The 16S rRNA gene sequence analysis revealed that strain CGII-59m2 belonged to the family and shared the highest pairwise similarity values with LMG 24012 (97.7 %), various species of the genus (97.3–97.0 %) and LMG 24812 (97.0 %). Cells of strain CGII-59m2 were rod-shaped, non-motile, and oxidase- and catalase-positive. The predominant fatty acids were Cω7, C, cyclo C and Cω7, the major respiratory quinone was Q-8, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid. The G+C content of the genomic DNA of strain CGII-59m2 was 62.3 mol%. The new bacterium can be distinguished from the closely related type strains based on its non-motile cells and its high Cω7 fatty acid content. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CGII-59m2 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is CGII-59m2 (=DSM 29806=NCAIM B.02615).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): activated sludge , Caenimicrobium , class Betaproteobacteria , family Alcaligenaceae and new genus
© 2017 IUMS
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2017-03-01
2024-04-16
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Caenimicrobium hargitense gen. nov., sp. nov., a new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge
Int J Syst Evol Microbiol 67, 627 (2017); https://doi.org/10.1099/ijsem.0.001674
/content/journal/ijsem/10.1099/ijsem.0.001674
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