%0 Journal Article %A Felföldi, Tamás %A Schumann, Peter %A Mentes, Anikó %A Kéki, Zsuzsa %A Máthé, István %A Tóth, Erika M %T Caenimicrobium hargitense gen. nov., sp. nov., a new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 3 %P 627-632 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001674 %K activated sludge %K Caenimicrobium %K family Alcaligenaceae %K new genus %K class Betaproteobacteria %I Microbiology Society, %X A new betaproteobacterium, CGII-59m2T, was isolated from an activated sludge bioreactor which treated landfill leachate. The 16S rRNA gene sequence analysis revealed that strain CGII-59m2T belonged to the family Alcaligenaceae and shared the highest pairwise similarity values with Parapusillimonas granuli LMG 24012T (97.7 %), various species of the genus Bordetella (97.3–97.0 %) and Candidimonas nitroreducens LMG 24812T (97.0 %). Cells of strain CGII-59m2T were rod-shaped, non-motile, and oxidase- and catalase-positive. The predominant fatty acids were C16 : 1ω7c, C16 : 0, cyclo C17 : 0 and C18 : 1ω7c, the major respiratory quinone was Q-8, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid. The G+C content of the genomic DNA of strain CGII-59m2T was 62.3 mol%. The new bacterium can be distinguished from the closely related type strains based on its non-motile cells and its high C16 : 1ω7c fatty acid content. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CGII-59m2T is considered to represent a novel species of a new genus, for which the name Caenimicrobium hargitense gen. nov., sp. nov. is proposed. The type strain is CGII-59m2T (=DSM 29806T=NCAIM B.02615T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001674