@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001698, author = "Tambong, James T. and Xu, Renlin and Bromfield, Eden S. P.", title = "Pseudomonas canadensis sp. nov., a biological control agent isolated from a field plot under long-term mineral fertilization", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "4", pages = "889-895", doi = "https://doi.org/10.1099/ijsem.0.001698", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001698", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Pseudomonas canadensis sp. nov.", keywords = "polar lipids", keywords = "biological control", keywords = "digital DNA-DNA hybridization (dDDH)", keywords = "ANIm", keywords = "genome sequencing", abstract = "The bacterial strain 2-92T, isolated from a field plot under long-term (>40 years) mineral fertilization, exhibited in vitro antagonistic properties against fungal pathogens. A polyphasic approach was undertaken to verify its taxonomic status. Strain 2-92T was Gram-reaction-negative, aerobic, non-spore-forming, motile by one or more flagella, and oxidase-, catalase- and urease-positive. The optimal growth temperature of strain 2-92T was 30 °C. 16S rRNA gene sequence analysis demonstrated that the strain is related to species of the genus Pseudomonas . Phylogenetic analysis of six housekeeping genes (dna A, gyr B, rec A, rec F, rpo B and rpo D) revealed that strain 2-92T clustered as a distinct and well separated lineage with Pseudomonas simiae as the most closely related species. Polar lipid and fatty acid compositions corroborated the taxonomic position of strain 2-92T in the genus Pseudomonas . Phenotypic characteristics from carbon utilization tests could be used to differentiate strain 2-92T from closely related species of the genus Pseudomonas . DNA–DNA hybridization values (wet laboratory and genome-based) and average nucleotide identity data confirmed that this strain represents a novel species. On the basis of phenotypic and genotypic characteristics, it is concluded that this strain represents a separate novel species for which the name Pseudomonas canadensis sp. nov. is proposed, with type strain 2-92T (=LMG 28499T=DOAB 798T). The DNA G+C content is 60.30 mol%.", }