RT Journal Article SR Electronic(1) A1 Zhao, Xin A1 Li, Danyang A1 Xu, Shuhong A1 Guo, Zhanghao A1 Zhang, Yan A1 Man, Lin A1 Jiang, Binhui A1 Hu, XiaominYR 2017 T1 Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., two phylogenetically closely related hydrogen-producing species isolated from lake sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 3 SP 710 OP 715 DO https://doi.org/10.1099/ijsem.0.001702 PB Microbiology Society, SN 1466-5034, AB Two novel anaerobic, mesophilic, biohydrogen-producing bacteria, designated strains ZGM211T and G1T, were isolated from lake sediment. 16S rRNA and ATP synthase beta subunit (atp D) gene sequences and phylogenetic analysis of strains ZGM211T and G1T revealed an affiliation to the genus Clostridium sensu stricto (cluster I of the clostridia), with Clostridium acetobutylicum as the closest characterized species, showing the same sequence similarity of 96.4 % to the type strain (98.9 % between the two isolates). Cells of the two strains were rod shaped. Growth occurred at 20–45 °C, pH 4.0–8.0 and NaCl concentrations up to 2 % (w/v). Grown on glucose, the main fermentation products were H2, CO2, acetate and butyrate. The major fatty acids were C14 : 0 and C16 : 0. The DNA G+C contents of strains ZGM211T and G1T were 40.7 and 41.5 mol%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic differences, strains ZGM211T (=CICC 24070T=BCRC 80950T) and G1T (=CICC 24069T=BCRC 80949T) are proposed as the type strains of novel species of the genus Clostridium with the names Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., respectively., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001702