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Abstract

A Gram-stain-negative, motile, non-spore-forming bacterium, designated strain Y4G10-17, was isolated from the saline–alkali farmland top soil, Inner Mongolia, northern China. Strain Y4G10-17 could grow at 4–45 °C (with 30 °C as the optimal temperature), pH 6.0–12.0 (optimal at pH 9.0) and in the presence of 1.0–12.0 % (w/v) NaCl (optimal at 4.0–6.0 %). Phylogenetic analysis based on the eight different copies of the 16S rRNA gene sequences revealed that strain Y4G10-17 shared the highest sequence similarity with CF12-14, 97.93–98.66 %, and lower than 97.0 % sequence similarity with all other type strains. Its major cellular fatty acids contained iso-C, iso-C, summed feature 9 (iso-Cω9 and/or C 10-methyl), iso-C F, iso-C 3-OH and summed feature 3 (iso-C 2-OH and/or Cω7). Q-8 was the predominantubiquinone. The major polar lipids of strain Y4G10-17 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unknown lipids and one unknown aminolipid. The genomic DNA G+C content was 49.3 mol%. DNA–DNA hybridization revealed that strain Y4G10-17 showed 20.2±5 % genomic DNA relatedness with its close relative CF12-14. Based on the phenotypic, phylogenetic and genotypic characteristics, strain Y4G10-17 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is Y4G10-17 (=CGMCC 1.15759=KCTC 52381).

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2017-03-01
2024-03-28
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