@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001727, author = "Chaudhary, Dhiraj Kumar and Kim, Jaisoo", title = "Roseomonas nepalensis sp. nov., isolated from oil-contaminated soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "4", pages = "981-987", doi = "https://doi.org/10.1099/ijsem.0.001727", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001727", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Acetobacteraceae", keywords = "oil-contaminated soil", keywords = "Proteobacteria", keywords = "Roseomonas nepalensis sp. nov.", abstract = "A wine-red-coloured, Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain G-3-5T, was isolated from oil-contaminated soil of Biratnagar, Morang, Nepal, during a study of oil-utilizing bacteria. This strain was catalase-negative and oxidase-positive. It was able to grow at 10–37 °C, at pH 6.0–10.0 and with 0.02–1.02 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain G-3-5T belongs to the genus Roseomonas and is closely related to Roseomonas vinacea CPCC 100056T (97.81 % sequence similarity), Roseomonas aerilata 5420S-30T (96.68 %), Roseomonas pecuniae N75T (96.15 %), ‘ Roseomonas aceris ’ R-1 (95.75 %) and Roseomonas rosea 173/96T (95.30 %). The only respiratory quinone was ubiquinone-10. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major fatty acids of strain G-3-5T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, C18 : 0 and C18 : 1 2-OH. The genomic DNA G+C content of this novel strain was 68.3 mol%. The DNA–DNA relatedness between strain G-3-5T and Roseomonas. vinacea KACC 13934T was 26.3 %. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain G-3-5T represents a novel species of the genus Roseomonas , for which the name Roseomonas nepalensis sp. nov. is proposed. The type strain is G-3-5T (=KEMB 9005-416T=KACC 18908T=JCM 31470T).", }