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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 5

sp. nov. isolated from rhizosphere soil Free

Abstract

A bacterial strain designated RA9 was isolated from a root of in Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate into the genus with its closest relatives being R-6514 and R-7190 with 98.2 % similarity in both cases. DNA–DNA hybridization studies showed mean relatedness values of 29 and 30 %, respectively, between strain RA9 and the type strains of and . Cells of the isolate were Gram-stain-positive, motile, sporulating rods. Catalase and oxidase were positive. Gelatin, starch and casein were not hydrolysed. Menaquinone MK-7 was the only menaquinone detected and iso-C and anteiso-C were the major fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, one unidentifed glycolipid and one unidentified lipid. -Diaminopimelic acid was detected in the peptidoglycan. The DNA G+C content was 43.1 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain RA9 should be considered as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RA9 (=LMG 29736=CECT 9170).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus , Cistus ladanifer , rhizosphere and soil
© 2017 IUMS
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2017-05-01
2024-04-16
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Bacillus terrae sp. nov. isolated from Cistus ladanifer rhizosphere soil
Int J Syst Evol Microbiol 67, 1478 (2017); https://doi.org/10.1099/ijsem.0.001742
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