@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001742, author = "Díez-Méndez, Alexandra and Rivas, Raúl and Mateos, Pedro F and Martínez-Molina, Eustoquio and Santín, Primitivo Julio and Sánchez-Rodríguez, Juan Antonio and Velázquez, Encarna", title = "Bacillus terrae sp. nov. isolated from Cistus ladanifer rhizosphere soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "5", pages = "1478-1481", doi = "https://doi.org/10.1099/ijsem.0.001742", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001742", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "rhizosphere", keywords = "Cistus ladanifer", keywords = "Bacillus", keywords = "soil", abstract = "A bacterial strain designated RA9T was isolated from a root of Cistus ladanifer in Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate into the genus Bacillus with its closest relatives being Bacillus fortis R-6514T and Bacillus fordii R-7190T with 98.2 % similarity in both cases. DNA–DNA hybridization studies showed mean relatedness values of 29 and 30 %, respectively, between strain RA9T and the type strains of B. fortis and B. fordii . Cells of the isolate were Gram-stain-positive, motile, sporulating rods. Catalase and oxidase were positive. Gelatin, starch and casein were not hydrolysed. Menaquinone MK-7 was the only menaquinone detected and iso-C15 : 0 and anteiso-C15 : 0 were the major fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, one unidentifed glycolipid and one unidentified lipid. meso-Diaminopimelic acid was detected in the peptidoglycan. The DNA G+C content was 43.1 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain RA9T should be considered as representing a novel species of the genus Bacillus , for which the name Bacillus terrae sp. nov. is proposed. The type strain is RA9T (=LMG 29736T=CECT 9170T).", }