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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 5

sp. nov., isolated from a sludge sample of a biofilm reactor Free

Abstract

A polyphasic taxonomic study was carried out on strain EBR-4-2 isolated from a biofilm reactor in Korea. Cells of the strain were Gram-stain-positive, non-spore-forming, non-motile and rod-shaped. Comparative 16S rRNA gene sequence studies showed the clear affiliation of this strain to the , and it had the highest pairwise sequence similarities with EGI 60002 (98.7 %), CF5-4 (96.3 %) and DSM 3133 (96.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage with the genus . The major fatty acids were identified as C anteiso, C, C N alcohol, C anteiso A and C iso. The major cellular polar lipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol and glycolipid. The peptidoglycan type was A4β containing -Orn–-Glu. The whole-cell-wall sugars were glucose and ribose. The respiratory quinone was identified as menaquinone MK-10(H), and the genomic DNA G+C content was determined to be 74.8 mol %. Based on evidence from this polyphasic study, it is proposed that strain EBR-4–2 should be designated as representing a novel species named sp. nov. The type stain is EBR-4-2 (=KCTC 33604=JCM 30447).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinotalea , Actinotalea caeni , biofilm reactor and EBR-4-2
© 2017 IUMS
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2017-05-01
2024-04-25
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Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor
Int J Syst Evol Microbiol 67, 1595 (2017); https://doi.org/10.1099/ijsem.0.001769
/content/journal/ijsem/10.1099/ijsem.0.001769
/content/journal/ijsem/10.1099/ijsem.0.001769
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