@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001769, author = "Jin, Long and Ko, So-Ra and Lee, Chang Soo and Ahn, Chi-Yong and Lee, Jung-Sook and Lee, Keun Chul and Oh, Hee-Mock and Lee, Hyung-Gwan", title = "Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "5", pages = "1595-1599", doi = "https://doi.org/10.1099/ijsem.0.001769", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001769", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Actinotalea", keywords = "Actinotalea caeni", keywords = "EBR-4-2", keywords = "biofilm reactor", abstract = "A polyphasic taxonomic study was carried out on strain EBR-4-2T isolated from a biofilm reactor in Korea. Cells of the strain were Gram-stain-positive, non-spore-forming, non-motile and rod-shaped. Comparative 16S rRNA gene sequence studies showed the clear affiliation of this strain to the Actinobacteria , and it had the highest pairwise sequence similarities with Actinotalea suaedae EGI 60002T (98.7 %), Actinotalea ferrariae CF5-4T (96.3 %) and Actinotalea fermentans DSM 3133T (96.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage with the genus Actinotalea . The major fatty acids were identified as C15 : 0 anteiso, C16 : 0, C16 : 0 N alcohol, C15 : 1 anteiso A and C15 : 0 iso. The major cellular polar lipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol and glycolipid. The peptidoglycan type was A4β containing l-Orn–d-Glu. The whole-cell-wall sugars were glucose and ribose. The respiratory quinone was identified as menaquinone MK-10(H4), and the genomic DNA G+C content was determined to be 74.8 mol %. Based on evidence from this polyphasic study, it is proposed that strain EBR-4–2T should be designated as representing a novel species named Actinotalea caeni sp. nov. The type stain is EBR-4-2T (=KCTC 33604T=JCM 30447T).", }