1887

Abstract

A novel bacterial strain, MVW-6, was isolated from a freshwater spring in Taiwan and characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-6 belonged to the genus and showed the highest levels of sequence similarity to CL-GP79 (98.2 % 16S rRNA gene sequence similarity) and MIB-4 (97.4 %). Cells of strain MVW-6 were Gram-stain-negative, aerobic, non-motile rods that were covered by large capsules and formed light pink colonies. Growth occurred at 15–37 °C (optimum 15–25 °C), at pH 5–9 (optimum pH 7) and with 0–0.5 % (w/v) NaCl (optimum 0 %). Strain MVW-6 contained iso-C, summed feature 3 (Cω7 and/or Cω6), Cω5 and C as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, five unidentified aminophospholipids, one unidentified glycolipid, one unidentified phospholipid, one unidentified aminolipid and two unidentified lipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 39.5 mol%. DNA–DNA hybridization values for strain MVW-6 with CL-GP79 and MIB-4 were less than 50 %. On the basis of the phylogenetic inference and phenotypic data, strain MVW-6 a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MVW-6 (=BCRC 80975=LMG 29555=KCTC 33800).

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2017-05-01
2024-03-28
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References

  1. Larkin JM, Williams PM, Taylor R. Taxonomy of the genus Microcyclus Orskov 1928: reintroduction and emendation of the genus Spirosoma Migula 1894 and proposal of a new genus, Flectobacillus. Int J Syst Bacteriol 1977; 27:147–156 [View Article]
    [Google Scholar]
  2. Ramaprasad EV, Sasikala Ch, Ramana ChV. Flectobacillus rhizosphaerae sp. nov., isolated from the rhizosphere soil of Oryza sativa (L.), and emended description of the genus Flectobacillus. Int J Syst Evol Microbiol 2015; 65:3451–3456 [View Article][PubMed]
    [Google Scholar]
  3. Ludwig W, Euzéby J, Whitman WB. Taxonomic outlines of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. In Whitman W. (editor) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 4 Baltimore, MD: Williams & Wilkins; 2011 pp. 21–24
    [Google Scholar]
  4. Hwang CY, Cho BC. Flectobacillus lacus sp. nov., isolated from a highly eutrophic pond in Korea. Int J Syst Evol Microbiol 2006; 56:1197–1201 [View Article][PubMed]
    [Google Scholar]
  5. Sheu SY, Chiu TF, Cho NT, Chou JH, Sheu DS et al. Flectobacillus roseus sp. nov., isolated from freshwater in Taiwan. Int J Syst Evol Microbiol 2009; 59:2546–2551 [View Article][PubMed]
    [Google Scholar]
  6. Chen WM, Laevens S, Lee TM, Coenye T, de Vos P et al. Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 2001; 51:1729–1735 [View Article][PubMed]
    [Google Scholar]
  7. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
    [Google Scholar]
  8. Anzai Y, Kudo Y, Oyaizu H. The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. Int J Syst Bacteriol 1997; 47:249–251 [View Article][PubMed]
    [Google Scholar]
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  10. Cole JR, Wang Q, Cardenas E, Fish J, Chai B et al. The ribosomal database project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 2009; 37:D141–D145 [View Article][PubMed]
    [Google Scholar]
  11. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  12. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  13. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  14. Kimura M. The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; 1983 [CrossRef]
    [Google Scholar]
  15. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  16. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  17. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Zool 1969; 18:1–32 [View Article]
    [Google Scholar]
  18. Rzhetsky A, Nei M. Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 1993; 10:1073–1095[PubMed]
    [Google Scholar]
  19. Felsenstein J. PHYLIP (Phylogeny Inference Package), Version 3.5c Distributed by the author Seattle, USA: Department of Genome Sciences, University of Washington; 1993
    [Google Scholar]
  20. Powers EM. Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 1995; 61:3756–3758[PubMed]
    [Google Scholar]
  21. Schlegel HG, Lafferty R, Krauss I. The isolation of mutants not accumulating poly-β-hydroxybutyric acid. Archiv für Mikrobiologie 1970; 71:283–294 [View Article]
    [Google Scholar]
  22. Spiekermann P, Rehm BH, Kalscheuer R, Baumeister D, Steinbüchel A. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 1999; 171:73–80 [View Article][PubMed]
    [Google Scholar]
  23. Reichenbach H. The order Cytophagales. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH. et al (editors) The Prokaryotes, a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd ed. New York, NY: Springer; 1992 pp. 3631–3675
    [Google Scholar]
  24. Schmidt K, Connor A, Britton G. Analysis of pigments: carotenoids and related polyenes. In Goodfellow M, AG O’Donnell. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994 pp. 403–461
    [Google Scholar]
  25. Breznak JA, Costilow RN. Physicochemical factors in growth. In Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR et al. (editors) Methods for General and Molecular Bacteriology, 3rd ed. Washington, DC: American Society for Microbiology; 2007 pp. 309–329
    [Google Scholar]
  26. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematic. In Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR. et al (editors) Methods for General and Molecular Bacteriology, 3rd ed. Washington, DC: American Society for Microbiology; 2007 pp. 330–393
    [Google Scholar]
  27. Wen CM, Tseng CS, Cheng CY, Li YK. Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. Biotechnol Appl Biochem 2002; 35:213–219 [View Article][PubMed]
    [Google Scholar]
  28. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  29. Nokhal T-H, Schlegel HG. Taxonomic study of Paracoccus denitrificans. Int J Syst Bacteriol 1983; 33:26–37 [View Article]
    [Google Scholar]
  30. Chen WM, Lin KR, Young CC, Sheu SY. Flectobacillus fontis sp. nov., isolated from a freshwater spring. Int J Syst Evol Microbiol 2017; 67:336–342 [View Article][PubMed]
    [Google Scholar]
  31. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  32. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [CrossRef]
    [Google Scholar]
  33. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  34. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994 pp. 121–161
    [Google Scholar]
  35. Collins MD. Isoprenoid quinones. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994 pp. 265–309
    [Google Scholar]
  36. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
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