1887

Abstract

A phosphate-solubilizing bacterium, designated CPCC 100624, was isolated from a freshwater reservoir in south-west China. The 16S rRNA gene sequence comparison of strain CPCC 100624 with the available sequences in the GenBank database showed that the isolate was closely related to members of the genus In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 100624 formed a stable phylogenetic subclade with MIMBbqt12 within the genus , which indicated that strain CPCC 100624 could be identified as a member of the genus The strain grew at 10–40 °C (optimum 30 °C), at pH 6.5–8.0 (optimum pH 7.0–7.5) and in the presence of 0–5 % (w/v) NaCl (optimum 0–1 %). MK-7 was detected as the main menaquinone, with a minor amount of MK-7(H) in its menaquinone system. Cells contained summed feature 3 (Cω6 and/or Cω7), Cω5 and iso-C as the major fatty acids. The polar lipids of strain CPCC 100624 contained phosphatidylethanolamine, one unidentified aminolipid, two aminophospholipids and three unidentified lipids. The genomic DNA G+C content was 49.3 mol%. On the basis of the above taxonomic data and differences in physiological characteristics from the closely related type strains, strain CPCC 100624 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CPCC 100624 (=NBRC 111852=DSM 101771).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001778
2017-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3144.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001778&mimeType=html&fmt=ahah

References

  1. Migula W. Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1894; 1:235–238
    [Google Scholar]
  2. Kim SJ, Ahn JH, Weon HY, Hong SB, Seok SJ et al. Spirosoma aerophilum sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 2016; 66:2342–2346 [View Article][PubMed]
    [Google Scholar]
  3. Yang SS, Tang K, Zhang X, Wang J, Wang X et al. Spirosoma soli sp. nov., isolated from biological soil crusts. Int J Syst Evol Microbiol 2016; 66:5568–5574 [View Article][PubMed]
    [Google Scholar]
  4. Joo E-S, Kim E-B, Jeon S-H, Srinivasan S, Kim M-K. Spirosoma swuense sp. nov., isolated from wet soil. Int J Syst Evol Microbiol 2017; 67:532–536 [View Article][PubMed]
    [Google Scholar]
  5. Ahn JH, Weon HY, Kim SJ, Hong SB, Seok SJ et al. Spirosoma oryzae sp. nov., isolated from rice soil and emended description of the genus Spirosoma. Int J Syst Evol Microbiol 2014; 64:3230–3234 [View Article][PubMed]
    [Google Scholar]
  6. Finster KW, Herbert RA, Lomstein BA. Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high arctic permafrost soil, and emended description of the genus Spirosoma. Int J Syst Evol Microbiol 2009; 59:839–844 [View Article][PubMed]
    [Google Scholar]
  7. Smibert RM, Krieg NL. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  8. Zhang YQ, Yu LY, Wang D, Liu HY, Sun CH et al. Roseomonas vinacea sp. nov., a Gram-negative coccobacillus isolated from a soil sample. Int J Syst Evol Microbiol 2008; 58:2070–2074 [View Article][PubMed]
    [Google Scholar]
  9. Zhang BH, Salam N, Cheng J, Li HQ, Yang JY et al. Modestobacter lacusdianchii sp. nov., a phosphate-solubilizing actinobacterium with ability to promote microcystis growth. PLoS One 2016; 11:e0161069 [View Article][PubMed]
    [Google Scholar]
  10. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  11. Dittmer JC, Lester RL. A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 1964; 5:126–127[PubMed]
    [Google Scholar]
  12. Lee JS, Shin YK, Yoon JH, Takeuchi M, Pyun YR et al. Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Evol Microbiol 2001; 51:1491–1498 [View Article][PubMed]
    [Google Scholar]
  13. Kroppenstedt RM. Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20) New York, NY: Academic Press; 1985 pp. 173–199
    [Google Scholar]
  14. Meier A, Kirschner P, Schröder KH, Wolters J, Kroppenstedt RM et al. Mycobacterium intermedium sp. nov. Int J Syst Bacteriol 1993; 43:204–209 [View Article][PubMed]
    [Google Scholar]
  15. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  16. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  18. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  19. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  20. Kimura M. The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; 1983 [CrossRef]
    [Google Scholar]
  21. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Zool 1969; 18:1–32 [View Article]
    [Google Scholar]
  22. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  23. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  24. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
    [Google Scholar]
  25. Chang X, Jiang F, Wang T, Kan W, Qu Z et al. Spirosoma arcticum sp. nov., isolated from high arctic glacial till. Int J Syst Evol Microbiol 2014; 64:2233–2237 [View Article][PubMed]
    [Google Scholar]
  26. Fries J, Pfeiffer S, Kuffner M, Sessitsch A. Spirosoma endophyticum sp. nov., isolated from Zn- and Cd-accumulating Salix caprea. Int J Syst Evol Microbiol 2013; 63:4586–4590 [View Article][PubMed]
    [Google Scholar]
  27. Hatayama K, Kuno T. Spirosoma fluviale sp. nov., isolated from river water. Int J Syst Evol Microbiol 2015; 65:3447–3450 [View Article][PubMed]
    [Google Scholar]
  28. Ten LN, Xu JL, Jin FX, Im WT, Oh HM et al. Spirosoma panaciterrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 2009; 59:331–335 [View Article][PubMed]
    [Google Scholar]
  29. Baik KS, Kim MS, Park SC, Lee DW, Lee SD et al. Spirosoma rigui sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 2007; 57:2870–2873 [View Article][PubMed]
    [Google Scholar]
  30. Kim DU, Lee H, Kim SG, Ahn JH, Park SY et al. Spirosoma aerolatum sp. nov., isolated from a motor car air conditioning system. Int J Syst Evol Microbiol 2015; 65:4003–4007 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001778
Loading
/content/journal/ijsem/10.1099/ijsem.0.001778
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error