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Volume 67, Issue 5

sp. nov., an endophyte isolated from the roots of Free

Abstract

A facultatively anaerobic, Gram-stain-negative, rod-shaped, nitrogen-fixing, endophytic bacterial strain designated MP23 was isolated from the roots of growing in Chilika Lagoon, Odisha, India. Strain MP23 was slightly halophilic, and the optimal NaCl concentration and temperature for growth were 1 % and 30 °C, respectively. On the basis of 16S rRNA gene sequence similarities, strain MP23 was affiliated to the family and most closely related to KCTC 42181 and DSM 19579 with 99.71 % similarity, followed by subsp. DSM 9220 (97.22 %), LMG 26250 (97.14 %) and subsp. DSM 14847 (97 %). Sequence analysis of 16S rRNA, , and genes showed that strain MP23 formed a phylogenetic cluster with KCTC 42181 and DSM 19579 indicating that it belongs to the genus . The major cellular fatty acids were C, Cω6 and/or Cω7, Cω6 and/or Cω7, C, C 3-OH and/or iso-C I and C cyclo. Polar lipids of strain MP23 consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 50.3 mol%. Based on experimental DNA–DNA hybridization values and average nucleotide identity derived from comparison of whole-genome sequences, strain MP23 could be distinguished from its closest neighbours. We therefore conclude that strain MP23 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is MP23 (=DSM 100250=KCTC 42580).

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Keyword(s): Chilika Lagoon , Mangrovibacter phragmitis sp. nov. , Phragmites karka and Polyphasic taxonomy
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2017-05-01
2024-04-24
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Mangrovibacter phragmitis sp. nov., an endophyte isolated from the roots of Phragmites karka
Int J Syst Evol Microbiol 67, 1228 (2017); https://doi.org/10.1099/ijsem.0.001789
/content/journal/ijsem/10.1099/ijsem.0.001789
/content/journal/ijsem/10.1099/ijsem.0.001789
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