1887

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Volume 67, Issue 5

sp. nov., isolated from soil of ginseng rhizosphere Free

Abstract

A Gram-stain-negative, aerobic, non-motile, non-spore-forming, yellow and rod-shaped bacterium, designated strain CR164, was isolated from the rhizosphere soil of a ginseng field at Geumsan in Korea. CR164 grew at between 15 and 37 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.0) and at salinities of 0–1.0 % (w/v) NaCl, growing optimally in the absence of NaCl. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that CR164 represents a member of the genus , showing the highest sequence similarity to MJ01 (98.5 %), Gsoil 3054 (98.4 %), LCS2 (98.3 %), RP5557 (98.1 %), 2APBS1 (98.0 %), Jip2 (97.6 %), DCY45 (97.3 %) and ‘’ BJQ-6 (97.0 %). The major fatty acids were iso-Cω9 (21.8 %), iso-C (12.1 %), iso-C (11.9 %) and iso-C (11.1 %). The predominant ubiquinone was Q-8. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The G+C content of the genomic DNA was 62.3 mol%. DNA–DNA relatedness between CR164 and the type strains of eight other species of the genus ranged from 51 to 9 %. On the basis of the polyphasic analysis, CR164 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CR164 (=KACC 18699=NBRC 111845).

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Keyword(s): ginseng , rhizosphere and Rhodanobacter rhizosphaerae sp. nov.
© 2017 IUMS
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2017-05-01
2024-04-19
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Rhodanobacter rhizosphaerae sp. nov., isolated from soil of ginseng rhizosphere
Int J Syst Evol Microbiol 67, 1387 (2017); https://doi.org/10.1099/ijsem.0.001825
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