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Sanguibacter gelidistatuariae sp. nov., a novel psychrotolerant anaerobe from an ice sculpture in Antarctica, and emendation of descriptions for the family Sanguibacteraceae, the genus Sanguibacter and species S. antarcticus, S. inulinus, S. kedieii, S. marinus, S.soli and S. suarezii
- Authors: Elena V. Pikuta1, Zhe Lyu2, Melissa D. Williams3, Nisha B. Patel4, Yuchen Liu5, Richard Brice Hoover6, Hans-Jürgen Busse7, Paul A. Lawson8, William B. Whitman9
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1 1Athens State University 2 2Microbiology Department, University of Georgia in Athens, Athens GA, 30602 USA 3 3Biology Department, University of Alabama in Huntsville, Huntsville AL, 35899 USA; current: Genome Sequencing Center, HudsonAlpha Institute for Biotechnology, Huntsville AL, 35806 USA 4 4University of Oklahoma 5 5Department of Biological Sciences, Louisiana State University, Baton Rouge LA, 70803 USA 6 6Mathematical, Computer & Natural Sciences Dpt., Athens State University, Athens AL, 35611 USA 7 7Institut für Mikrobiologie, Veterinarmedizinische Universität Wien, A-1210 Wien, Austria 8 8Dept. of Microbiology & Plant Biology, University of Oklahoma, Norman OK, 73019 USA 9 9Microbiology Department, University of Georgia in Athens, Athens GA, 30602 USA
- First Published Online: 01 February 2017, International Journal of Systematic and Evolutionary Microbiology doi: 10.1099/ijsem.0.001838
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Sanguibacter gelidistatuariae sp. nov., a novel psychrotolerant anaerobe from an ice sculpture in Antarctica, and emendation of descriptions for the family Sanguibacteraceae, the genus Sanguibacter and species S. antarcticus, S. inulinus, S. kedieii, S. marinus, S.soli and S. suarezii, Page 1 of 1< Previous page | Next page > /docserver/preview/fulltext/ijsem/ijsem_pap.001838.rev1.zip/ijsem.0.001838-1.gif
A novel psychrotolerant bacterium strain ISLP-3T was isolated from a sample of naturally formed ice sculpture on the shore of Lake Podprudnoye in Antarctica. Cells were motile, stained Gram positive, non-spore-forming, straight or slightly curved rods with the shape of a baseball bat. The new isolate was facultatively anaerobic and catalase positive. Growth occurred at 3-35 ºC with an optimum at 24 °C, 0-2 % w/v NaCl with an optimum at 0.3 % and pH 6.2 - 9.5 with an optimum 7.5. Strain ISLP-3T grew on several carbon sources, with the best growth on D-cellobiose. The isolate possessed ureolytic activity but growth was inhibited by urea. The strain was sensitive to: ampicillin, gentamycin, kanamycin rifampicin, tetracycline and chloramphenicol. Major fatty acids were: C 15:0 anteiso, C 16:0 iso, C 16:0, C14:0 and C15:0 iso. The predominant menaquinone was MK-9(H4). The genomic G+C content was 69.5 mol%.
The 16S rRNA gene possessed 99 % sequence similarity to the gene of Sanguibacter suarezii ST-26T, but their recA genes demonstrated ≤91 % sequence similarity, suggesting that this isolate represented a novel species within genus Sanguibacter. This conclusion was supported by ANI, which was ≤91 % to the most closely related strain. The name Sanguibacter gelidistatuariae sp. nov. is proposed for the new species with the type strain ISLP-3T(= ATCC TSD-17T, = DSM 100501T, = JCM 30887T). Complete genome draft sequence of ISLP-3T was deposited under IMG OID 2657245272. Emendments of related taxa have been made based on experimental data of our comparative analysis.
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