RT Journal Article SR Electronic(1) A1 Sripreechasak, Paranee A1 Phongsopitanun, Wongsakorn A1 Supong, Khomsan A1 Pittayakhajonwut, Pattama A1 Kudo, Takuji A1 Ohkuma, Moriya A1 Tanasupawat, SomboonYR 2017 T1 Nonomuraea rhodomycinica sp. nov., isolated from peat swamp forest soil JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 6 SP 1683 OP 1687 DO https://doi.org/10.1099/ijsem.0.001843 PB Microbiology Society, SN 1466-5034, AB The taxonomic position of an actinomycete, strain NR4-ASC07T, isolated from a soil sample collected from Sirindhorn peat swamp forest, Narathiwat Province, Thailand, was clarified using a polyphasic approach. On the basis of morphological and chemotaxonomic characteristics, it was classified among the members of the genus Nonomuraea . It produced tightly closed spiral spore chains on aerial mycelium as well as forming a pseudosporangium. Whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, ribose, madurose and mannose. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, unknown ninhydrin-positive phosphoglycolipids and unknown glycolipid. Menaquiones were MK-9(H4), MK-9(H0), MK-9(H2), MK-10(H4) and MK-9(H6). Predominant cellular fatty acids were iso-C16 : 0, C17 : 0 10-methyl, C16 : 0, C17 : 1ω8c, C16 : 0 2-OH and iso-C15 : 0. The phylogenetic tree reconstructed on the basis of 16S rRNA gene sequences showed that the strain fell within the clade containing Nonomuraea muscovyensis FMN03T, Nonomuraea roseoviolacea subsp. roseoviolacea NBRC 14098T and Nonomuraea roseoviolacea subsp. carminata NBRC 15903T. The DNA–DNA relatedness and phenotypic data supported that strain NR4-ASC07T was clearly distinguished from the closely related species and represents a novel species of the genus Nonomuraea for which the name Nonomuraea rhodomycinica sp. nov. is proposed. The type strain is NR4-ASC07T (=NBRC 112327T=TISTR 2465T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001843