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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 7

sp. nov., isolated from surface seawater Free

Abstract

A bacterial strain, designated 25B10_4, was isolated from surface seawater of the Bering Sea and characterized using a polyphasic taxonomic approach. Cells of strain 25B10_4 were Gram-stain-negative, motile and short-rod-shaped. Growth was observed at 10–43 °C (optimum 28 °C), at pH 6–9 (optimum pH 7) and with 0–12 % NaCl (optimum 3–4 %, w/v). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 25B10_4 formed an independent branch within the genus , sharing high similarities with four related type strains, 13D2W-2 (98.9 %), TL 2 (98.5 %), DLFJ1-1 (98.4 %) and DT23-4 (96.9 %), and low similarities (less than 96.5 %) with other type strains within the family . The major fatty acid was identified as summed feature 8 (Cω6 and/or Cω7). The quinone was Q-10. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, glycolipid and five unidentified phospholipids. The DNA G+C content was 63.8 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between strain 25B10_4 and the four above-mentioned type strains were, respectively, 20.5–25.7 % and 77.8–82.4 %. The phenotypic, phylogenetic and chemotaxonomic analyses clearly indicated that strain 25B10_4 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 25B10_4 (=MCCC 1A07302=LMG 29614).

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  • Accepted:
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Keyword(s): a polyphasic taxonomy approach , dDDH , surface seawater and Thioclava nitratireducens
© 2017 IUMS
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2017-07-01
2024-04-19
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Thioclava nitratireducens sp. nov., isolated from surface seawater
Int J Syst Evol Microbiol 67, 2109 (2017); https://doi.org/10.1099/ijsem.0.001844
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