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Pyrobaculum igneiluti sp. nov., a novel anaerobic hyperthermophilic archaeon that reduces thiosulfate and ferric iron
- Authors: Jerry Y. Lee1, Brenda Iglesias2, Caleb E. Chu3, Daniel J. P. Lawrence4, Edward J. Crane III5
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1 1Pomona College 2 2Pomona College 3 3Pomona College 4 4Pomona College 5 5Pomona College
- First Published Online: 02 February 2017, International Journal of Systematic and Evolutionary Microbiology doi: 10.1099/ijsem.0.001850
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A novel anaerobic, hyperthermophilic archaeon was isolated from a mud volcano in the Salton Sea geothermal system in Southern California, USA. The isolate, named strain 521T, grew optimally at 90°C, pH 5.5-7.3, and 0-2.0% w/v NaCl, with a generation time of 10 hours under optimal conditions. Cells were rod-shaped and nonmotile, ranging from 2-7 μm in length. Strain 521T grew only in the presence of thiosulfate and/or Fe(III) (ferrihydrite) as terminal electron acceptors in strictly anaerobic conditions, and preferred protein-rich compounds as energy sources, although the isolate was capable of chemolithoautotrophic growth. 16S rRNA gene sequence analysis places this isolate within the crenarchaeal Pyrobaculum genus. To our knowledge, this is the first Pyrobaculum strain to be isolated from an anaerobic mud volcano and to reduce only either thiosulfate or ferric iron. An in silico genome-to-genome distance calculator reported <25% DDH between strain 521T and eight other Pyrobaculum species. Due to its genotypic and phenotypic differences, we conclude that strain 521T (DSM 103086, ATCC TSD-56) is a new species for which the name Pyrobaculum igneiluti sp. nov. is proposed.
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