1887

Abstract

The Gram-reaction-negative, aerobic, white- to pale-yellow-coloured and rod-shaped bacterium with a single polar flagellum or a stalk, designated strain 7F14, was isolated from rhizosphere soil of cultivated watermelon () collected from Hefei, China. Growth of strain 7F14 was observed at pH 6.0–9.0, 10–30 °C and in the presence of 0–1 % (w/v) NaCl. Cells were catalase-negative and oxidase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 7F14 formed a phyletic lineage within the genus of the family and showed the highest 16S rRNA gene sequence similarities to ATCC 15253 (98.66 %), ATCC 21756 (98.27 %), CB51 (97.92 %) and RHGG3 (97.44 %). The G+C content of the genomic DNA was 68.6 mol%. Strain 7F14 contained Q-10 as the sole ubiquinone and 11-methyl Cω7, Cω7, C and summed feature 3 (Cω7 and/or iso-C 2-OH) as the major fatty acids. The polar lipids profile consisted of phosphatidylglycerol, an unknown phosphoglycolipid, five unknown glycolipids, an unknown phospholipid and three unknown lipids. DNA–DNA relatedness values to the most closely related type strains DSM 4730 and DSM 7131 were 26.0 and 19.7 %, respectively. Based on unique phenotypic traits, and phylogenetic, chemotaxonomic and DNA–DNA hybridization results, strain 7F14 should be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 7F14 (=CGMCC 1.15915=KCTC 52515).

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2017-06-01
2024-03-28
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