1887

Abstract

A Gram-staining-negative, rod-shaped, motile bacterium, designated WD12, was isolated from a rotten tree at Chungbuk National University, South Korea. WD12 grew optimally at 30–37 °C and pH 7.0–7.5 and could assimilate arbutin and potassium-5-ketogluconate. The major cellular fatty acid were iso-C, C, cyclo C, iso-C, summed features 3 (comprising Cω7/iso-C 2-OH) and anteiso-C. The major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was ubiquinone-8 (Q-8). The G+C content of the genomic DNA was 69.1 %. The results of phylogenetic and comparative analysis based on the 16S rRNA gene sequence indicated that WD12 formed a tight phylogenetic lineage with AMX 26B and 12-3 of the the genus in the family . Sequence similarity to other members of the genus ranged from 98.6 % ( AMX 26B) to 95.1 % ( CB-226). DNA–DNA relatedness between WD12 and eight type strains of species of the genus showing more than 97 % 16S rRNA sequence similarity were 6±0–26±1 %. On the basis of the evidence from this polyphasic study, WD12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WD12 (=KACC 15045=LMG 25968).

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2017-06-01
2024-03-28
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