1887

Abstract

A yellow-coloured, Gram-staining-negative, motile and rod shaped bacterium, designated strain R366, was isolated from forest soil of Kyonggi University, South Korea. It was able to grow at 15–42 °C, pH 6.0–10.0 and with 0–4 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain R366 belongs to the genus and is closely related to SC13E-S71 (98.72 % sequence similarity), S37 (98.51 %), Kp5.2 (98.29 %), RB2256 (98.15 %), Gsoli 250 (98.15 %) and JSS54 (98.01 %). The predominant respiratory quinone was ubiquinone-10, and the major polyamine was spermidine. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine and sphingoglycolipid. The predominant fatty acids of strain R366 were summed feature 8 (Cω7 and/or Cω6), summed feature 3 (Cω7 and/or Cω6), Cω6, C, Cω7 11-methyl and C 2-OH. The genomic DNA G+C content of this novel strain was 65.1 mol%. The DNA–DNA relatedness between strain R366 and DSM 25229, KCCM 41918, KCCM 41983, KACC 13918 and KACC 12341 was 51.7, 45.3, 39.0, 41.3 and 44.7 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain R366 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R366 (=KEMB 9005-451=KACC 19003=JCM 31675).

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2017-06-01
2024-03-29
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