1887

Abstract

[Lewin, 1969] was reclassified as NBRC 15981 was reclassified herein by using a polyphasic taxonomic approach. Cells of the strain were strictly aerobic, Gram-stain-negative, slender rods, which were motile by gliding. The major respiratory quinone was menaquinone-7 and the predominant (>5 %) cellular fatty acids were iso-C, iso-G-C, Cω7 and iso-C 3-OH. The polar lipid pattern indicated the presence of a phosphatidylethanolamine, several unidentified aminolipids, glycolipids and five unidentified polar lipids. The G+C content of the genomic DNA was 35.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NBRC 15981 clustered with members of the genus in the family of the phylum . Levels of DNA–DNA relatedness were less than 16 % between strain NBRC 15981 and the two closely related species, NBRC 15983 and NBRC 15989. Strain NBRC 15981 could be differentiated from these type strains in the genus based on the polar lipid pattern and the activity of α-chymotrypsin, as well as by α-glucosidase and β-glucosidase activity. On the basis of these results, NBRC 15981 is proposed as representing a novel species of the genus , named sp. nov. The type strain is JK11 (=NBRC 15981=NCIMB 1429).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001890
2017-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/1937.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001890&mimeType=html&fmt=ahah

References

  1. Nakagawa Y, Sakane T, Suzuki M, Hatano K. Phylogenetic structure of the genera Flexibacter, Flexithrix, and Microscilla deduced from 16S rRNA sequence analysis. J Gen Appl Microbiol 2002; 48:155–165 [View Article][PubMed]
    [Google Scholar]
  2. Lewin RA, Lounsbery DM. Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 1969; 58:145–170 [View Article][PubMed]
    [Google Scholar]
  3. Nedashkovskaya OI, Vancanneyt M, Kim SB, Bae KS. Reclassification of Flexibacter tractuosus (Lewin 1969) Leadbetter 1974 and 'Microscilla sericea' Lewin 1969 in the genus Marivirga gen. nov. as Marivirga tractuosa comb. nov. and Marivirga sericea nom. rev., comb. nov. Int J Syst Evol Microbiol 2010; 60:1858–1863 [View Article][PubMed]
    [Google Scholar]
  4. Lin CY, Zhang XY, Liu A, Liu C, Song XY et al. Marivirga atlantica sp. nov., isolated from seawater and emended description of the genus Marivirga. Int J Syst Evol Microbiol 2015; 65:1515–1519 [View Article][PubMed]
    [Google Scholar]
  5. Xu Y, Zhang R, Li Q, Liu K, Jiao N. Marivirga lumbricoides sp. nov., a marine bacterium isolated from the South China sea. Int J Syst Evol Microbiol 2015; 65:452–456 [View Article][PubMed]
    [Google Scholar]
  6. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  7. Barrow GI, Feltham RKA. Cowan and Steel's Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press; 1993 [CrossRef]
    [Google Scholar]
  8. Suzuki M, Nakagawa Y, Harayama S, Yamamoto S. Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 2001; 51:1639–1652 [View Article][PubMed]
    [Google Scholar]
  9. Smibert RM, Krieg NR. General characterization. In Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA. et al (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981 pp. 409–443
    [Google Scholar]
  10. Lau KW, Ng CY, Ren J, Lau SC, Qian PY et al. Owenweeksia hongkongensis gen. nov., sp. nov., a novel marine bacterium of the phylum 'Bacteroidetes'. Int J Syst Evol Microbiol 2005; 55:1051–1057 [View Article][PubMed]
    [Google Scholar]
  11. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32:1792–1797 [View Article][PubMed]
    [Google Scholar]
  12. Kumar S, Nei M, Dudley J, Tamura K. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 2008; 9:299–306 [View Article][PubMed]
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  14. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  15. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  16. Brosius J, Palmer ML, Kennedy PJ, Noller HF. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 1978; 75:4801–4805 [View Article][PubMed]
    [Google Scholar]
  17. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  18. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  19. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI technical note 101. Newark: MIDI Inc; 1990
    [Google Scholar]
  20. Nakagawa Y, Yamasato K. Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 1993; 139:1155–1161 [View Article][PubMed]
    [Google Scholar]
  21. Fautz E, Reichenbach H. A simple test for flexirubin-type pigments. FEMS Microbiol Lett 1980; 8:87–91 [View Article]
    [Google Scholar]
  22. Tamura T, Nakagaito Y, Nishii T, Hasegawa T, Stackebrandt E et al. A new genus of the order actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 1994; 44:193–203 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001890
Loading
/content/journal/ijsem/10.1099/ijsem.0.001890
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error