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Volume 67, Issue 6

sp. nov., isolated from pinnipeds, comprising subsp. subsp. nov. and subsp. subsp. nov. Free

Abstract

During independent diagnostic screenings of otariid seals in California (USA) and phocid seals in Scotland (UK), -like isolates, which differed from the established taxa of the genus , were cultured from abscesses and internal organs of different seal species. A polyphasic study was undertaken to determine the taxonomic position of these six isolates. The isolates were characterized by 16S rRNA gene and AtpA sequence analysis and by conventional phenotypic testing. The whole-genome sequences were determined for all isolates, and the average nucleotide identity (ANI) was determined. The isolates formed a separate phylogenetic clade, divergent from all other taxa of the genus and most closely related to . Although all isolates showed 100 % 16S rRNA gene sequence homology, AtpA and ANI analyses indicated divergence between the otariid isolates from California and the phocid isolates from Scotland, which warrants subspecies status for each clade. The two subspecies could also be distinguished phenotypically on the basis of catalase activity. This study shows clearly that the isolates obtained from pinnipeds represent a novel species within the genus , for which the name sp. nov. is proposed. Within this novel species, the Californian isolates represent a separate subspecies, for which the name subsp. subsp. nov. is proposed. The type strain for both this novel species and subspecies is RM17260 (=LMG 29472=CCUG 69570). The Scottish isolates represent another subspecies, for which the name subsp. subsp. nov. is proposed. The type strain of this subspecies is M302/10/6 (=LMG 29473=CCUG 68650).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): average nucleotide identity , Campylobacter , novel species , pinnipeds , urease and whole genome sequencing
© Microbiology Society
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2017-06-01
2024-04-25
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Campylobacter pinnipediorum sp. nov., isolated from pinnipeds, comprising Campylobacter pinnipediorum subsp. pinnipediorum subsp. nov. and Campylobacter pinnipediorum subsp. caledonicus subsp. nov.
Int J Syst Evol Microbiol 67, 1961 (2017); https://doi.org/10.1099/ijsem.0.001894
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