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Volume 67, Issue 6

sp. nov., an agar-digesting marine bacterium isolated from coastal seawater Free

Abstract

A novel Gram-stain-negative, facultatively anaerobic, yellowish and agar-digesting marine bacterium, designated strain QM50, was isolated from coastal seawater in an aquaculture site near Qingdao, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represented a member of the genus and exhibited the highest sequence similarity (97.4 %) to SMK-10. Average nucleotide identity (ANI) values based on draft genome sequences between strain QM50 and KCTC 12480 showed a relatedness of 72.0 % (ANIb) and 85.1 % (ANIm). Cells of strain QM50 were approximately 0.3–0.6×0.8–2.5 µm in size and motile by means of a polar flagellum. Growth occurred in the presence of 1.0–6.0 % (w/v) NaCl (optimum, 2.0–3.0 %), at pH 6.5–8.5 (optimum, pH 7.0) and at 4–37 °C (optimum, 28–30 °C). Strain QM50 was found to contain ubiquinone 8 (Q-8) as the predominant ubiquinone and summed feature 3 (Cω7 and/or iso-C 2-OH), C and Cω8 as the main cellular fatty acids. Phosphatidylethanolamine and phosphatidylglycerol were found to be major polar lipids. The DNA G+C content of strain QM50 was determined to be 35.7 mol%. On the basis of phylogenetic and phenotypic data, strain QM50 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is QM50 (=KCTC 52273=MCCC 1H00143).

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Keyword(s): 16S rDNA sequences , agar-digesting , Colwellia agarivorans sp. nov. and phylogenetic analysis
© 2017 IUMS
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2017-06-01
2024-04-18
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Colwellia agarivorans sp. nov., an agar-digesting marine bacterium isolated from coastal seawater
Int J Syst Evol Microbiol 67, 1969 (2017); https://doi.org/10.1099/ijsem.0.001897
/content/journal/ijsem/10.1099/ijsem.0.001897
/content/journal/ijsem/10.1099/ijsem.0.001897
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