1887

Abstract

Three strains (YIM-HL1107, YIM-HL1045, YIM-HL1112) representing a novel yeast species were isolated from surface water samples collected from the Caohai region of Dianchi Lake in Yunnan, south-western China. On the basis of morphological, physiological and biochemical characteristics and sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region, they were assigned to a novel species of the genus . The closest relative to the novel species was , but it showed 6.3 % nucleotide differences (34 nt substitutions out of 541 nt) in the D1/D2 region of the LSU rRNA gene and 9.3–9.6 % nucleotide differences (40–41 substitutions and 7–8 gaps out of 430 nt) in the ITS region. The name sp. nov. is proposed. The type strain is YIM-HL1107 (=CBS 14191=CCTCC AY 2015009), and the MycoBank number is MB 816297.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001908
2017-06-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/2014.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001908&mimeType=html&fmt=ahah

References

  1. Takashima M, Nakase T. Molecular phylogeny of the genus Cryptococcus and related species based on the sequences of 18S rDNA and internal transcribed spacer regions. Microbiol Cult Collect 1999; 15:35–47
    [Google Scholar]
  2. Scorzetti G, Fell JW, Fonseca A, Statzell-Tallman A. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2002; 2:495–517 [View Article][PubMed]
    [Google Scholar]
  3. Wang QM, Bai FY. Molecular phylogeny of basidiomycetous yeasts in the Cryptococcus luteolus lineage (Tremellales) based on nuclear rRNA and mitochondrial cytochrome b gene sequence analyses: proposal of Derxomyces gen. nov. and Hannaella gen. nov., and description of eight novel Derxomyces species. FEMS Yeast Res 2008; 8:799–814 [View Article][PubMed]
    [Google Scholar]
  4. Liu XZ, Wang QM, Theelen B, Groenewald M, Bai FY et al. Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 2015; 81:1–26 [View Article][PubMed]
    [Google Scholar]
  5. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 2015; 81:85–147 [View Article][PubMed]
    [Google Scholar]
  6. Landell MF, Brandão LR, Barbosa AC, Ramos JP, Safar SV et al. Hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil. Int J Syst Evol Microbiol 2014; 64:1970–1977 [View Article][PubMed]
    [Google Scholar]
  7. Kaewwichian R, Jindamorakot S, Am-In S, Sipiczki M, Limtong S. Hannaella siamensis sp. nov. and Hannaella phetchabunensis sp. nov., two new anamorphic basidiomycetous yeast species isolated from plants. Int J Syst Evol Microbiol 2015; 65:1297–1303 [View Article][PubMed]
    [Google Scholar]
  8. Surussawadee J, Jindamorakot S, Nakase T, Lee CF, Limtong S. Hannaella phyllophila sp. nov., a basidiomycetous yeast species associated with plants in Thailand and Taiwan. Int J Syst Evol Microbiol 2015; 65:2135–2140 [View Article][PubMed]
    [Google Scholar]
  9. Fonseca Á, Boekhout T, Fell JW. Cryptococcus Vuillemin. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp. 3864–4111
    [Google Scholar]
  10. Zhou J, Liu Y, Guo H, He D. Combining the SWAT model with sequential uncertainty fitting algorithm for streamflow prediction and uncertainty analysis for the Lake Dianchi Basin, China. Hydrol Process 2014; 28:521–533 [View Article]
    [Google Scholar]
  11. Yang Y, Zhao Q, Cui Y, Wang Y, Xie S et al. Spatio-temporal variation of sediment methanotrophic microorganisms in a large eutrophic lake. Microb Ecol 2016; 71:9–17 [View Article][PubMed]
    [Google Scholar]
  12. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
    [Google Scholar]
  13. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications San Diego, CA: Academic Press; 1990 pp. 315–322
    [Google Scholar]
  14. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
    [Google Scholar]
  15. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  18. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  20. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp. 87–110 [CrossRef]
    [Google Scholar]
  21. do Carmo-Sousa L, Phaff HJ. An improved method for the detection of spore discharge in the sporobolomycetaceae. J Bacteriol 1962; 83:434–435[PubMed]
    [Google Scholar]
  22. Nagahama T, Hamamoto M, Nakase T, Takaki Y, Horikoshi K. Cryptococcus surugaensis sp. nov., a novel yeast species from sediment collected on the deep-sea floor of Suruga Bay. Int J Syst Evol Microbiol 2003; 53:2095–2098 [View Article][PubMed]
    [Google Scholar]
  23. Dayo-Owoyemi I, Rodrigues A, Landell MF, Valente P, Mueller UG et al. Intraspecific variation and emendation of Hannaella kunmingensis. Mycol Prog 2013; 12:157–165 [View Article]
    [Google Scholar]
  24. Bai FY, Takashima M, Nakase T. Description of Bullera kunmingensis sp. nov., and clarification of the taxonomic status of Bullera sinensis and its synonyms based on molecular phylogenetic analysis. FEMS Yeast Res 2001; 1:103–109 [View Article][PubMed]
    [Google Scholar]
  25. Molnár O, Prillinger H. Cryptococcus zeae, a new yeast species associated with Zea mays. Microbiol Res 2006; 161:347–354 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001908
Loading
/content/journal/ijsem/10.1099/ijsem.0.001908
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error