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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 7

sp. nov., an endophytic bacterium isolated from stems Free

Abstract

Strain N5SSJ16, a Gram-negative-staining, non-spore-forming, rod-shaped, non-motile bacterium, was isolated from stems. The strain grew in the presence of 0–4 % (w/v) NaCl (optimum growth in the absence of NaCl), at pH 7–9 (optimum: pH 8) and at 12–50 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N5SSJ16 was related phylogenetically to 4M40 (96.5 %) and 4M29 (95.9 %). The cellular fatty acids were iso-C, summed feature 3 (Cω7 and/or Cω6), summed feature 9 (C 10-methyl and/or iso-Cω9) and iso-C 3-OH. MK-7 was the respiratory quinone. The main polar lipids were phosphatidylethanolamine, unidentified aminophospholipid and two unknown lipids. The G+C content of the DNA was 45.8 mol%. On the basis of chemotaxonomic, phylogenetic and phenotypic evidence, strain N5SSJ16 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N5SSJ16 (=ACCC 19928=KCTC 52416).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidetes , haloxylon ammodendron , new taxa and Parapedobacter deserti sp.nov
© 2017 IUMS
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2017-07-01
2024-04-19
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References

  1. Kim MK, Na JR, Cho DH, Soung NK, Yang DC. Parapedobacter koreensis gen. nov., sp. nov. Int J Syst Evol Microbiol 2007; 57:1336–1341 [View Article][PubMed]
     [Google Scholar]
  2. Kim MK, Kim YA, Kim YJ, Soung NK, Yi TH et al. Parapedobacter soli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2008; 58:337–340 [View Article][PubMed]
     [Google Scholar]
  3. Kim SJ, Weon HY, Kim YS, Yoo SH, Kim BY et al. Parapedobacter luteus sp. nov. and Parapedobacter composti sp. nov., isolated from cotton waste compost. Int J Syst Evol Microbiol 2010; 60:1849–1853 [View Article][PubMed]
     [Google Scholar]
  4. Zhao JK, Li XM, Zhang MJ, Jin JH, Jiang CY et al. Parapedobacter pyrenivorans sp. nov., isolated from a pyrene-degrading microbial enrichment, and emended description of the genus Parapedobacter . Int J Syst Evol Microbiol 2013; 63:3994–3999 [View Article][PubMed]
     [Google Scholar]
  5. Kumar R, Dwivedi V, Nayyar N, Verma H, Singh AK et al. Parapedobacter indicus sp. nov., isolated from hexachlorocyclohexane-contaminated soil. Int J Syst Evol Microbiol 2015; 65:129–134 [View Article][PubMed]
     [Google Scholar]
  6. Zhang X, Sun L, Ma X, Sui XH, Jiang R. Rhizobium pseudoryzae sp. nov., isolated from the rhizosphere of rice. Int J Syst Evol Microbiol 2011; 61:2425–2429 [View Article][PubMed]
     [Google Scholar]
  7. Li L, Sun L, Shi N, Liu L, Guo H et al. Chitinophaga cymbidii sp. nov., isolated from Cymbidium goeringii roots. Int J Syst Evol Microbiol 2013; 63:1800–1804 [View Article][PubMed]
     [Google Scholar]
  8. Dong XZ, Cai MY. (editors) Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press (in Chinese); 2001 pp. 370–398
     [Google Scholar]
  9. Sorokin DY. Is there a limit for high-pH life?. Int J Syst Evol Microbiol 2005; 55:1405–1406 [View Article][PubMed]
     [Google Scholar]
  10. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  11. Collins MD. Isoprenoid quinone analysis in bacterial classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp. 267–287
     [Google Scholar]
  12. Collins MD, Jones D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 1981; 45:316–354[PubMed]
     [Google Scholar]
  13. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  14. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp. 115–175
     [Google Scholar]
  15. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  16. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  17. Kimura M. The Neutral Theory of Molecular Evolution Cambridge: Cambridge UniversityPress; 1983 [CrossRef]
     [Google Scholar]
  18. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  19. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  20. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
     [Google Scholar]
  22. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–218 [View Article]
     [Google Scholar]
  23. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
     [Google Scholar]
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Parapedobacter deserti sp. nov., an endophytic bacterium isolated from Haloxylon ammodendron stems
Int J Syst Evol Microbiol 67, 2148 (2017); https://doi.org/10.1099/ijsem.0.001911
/content/journal/ijsem/10.1099/ijsem.0.001911
/content/journal/ijsem/10.1099/ijsem.0.001911
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