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Volume 67, Issue 7

sp. nov., an acetic acid bacterium isolated from the midgut of a honey bee Free

Abstract

As part of a study to investigate the microbial diversity in the intestine of , we isolated strain MRM1 from the midgut. MRM1 was a Gram-stain-negative, strictly aerobic, non-motile, non-spore forming and rod-shaped bacteria. Creamy beige-coloured colonies were circular with entire margins in Lactobacilli MRS agar. The strain grew at 25–37 °C (optimum, 30–37 °C) and at a pH range of 4.0 to 9.0 (optimum pH, 7.0–8.5). The strain tolerated 0–1 % (w/v) NaCl (optimal growth occurred in the absence of NaCl). On the basis of the results of a phylogenetic analysis based on the 16S rRNA gene sequences, we determined that MRM1 represents a member of the genus with the highest sequence similarity to LMG 28161 (98.8 %). The major quinone was Q10, and dominant fatty acids (>10 %) were Ccyclo ω8 (33.6 %), C (22.2 %), Cω7 (15.9 %) and C (12.5 %). The polar lipid profile of MRM1 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified phospholipid and four unidentified lipids. The DNA G+C content of MRM1 was 59.5 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, MRM1 represents a novel species of the genus , for which the name sp. nov. is proposed with the type strain MRM1 (=KCTC 52452=JCM 31623).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): alpha-2.2 , Apis mellifera , Bombella apis , gut bacteria and honey bee
© 2017 IUMS
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2017-07-01
2024-04-18
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Bombella apis sp. nov., an acetic acid bacterium isolated from the midgut of a honey bee
Int J Syst Evol Microbiol 67, 2184 (2017); https://doi.org/10.1099/ijsem.0.001921
/content/journal/ijsem/10.1099/ijsem.0.001921
/content/journal/ijsem/10.1099/ijsem.0.001921
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