1887

Abstract

A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strain, designated 2C-7, was isolated from symptomatic bark of a canker. Growth occurred between 10 and 37 °C and between pH 6 and 10, with optimal growth at 30 °C and pH 7.0–8.0. Growth was present under 0–8 % (w/v) salinity conditions (optimum 1–2 %). Growth occurred in the presence of 10 mM chromium (Cr). The major fatty acids (≥10 %) of the novel strain were anteiso-C, anteiso-C and iso-C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and two unknown lipids. The major respiratory quinone was menaquinone MK-11. The cell wall amino acids were 2,4-diaminobutyric acid, alanine, glutamic acid and glycine. Strain 2C-7 was most similar to subsp NAL101 (97.2 % 16S rRNA gene sequence similarity), ‘ F3-P9 (97.1 %) and L-1 (97.1 %). In the phylogenetic tree, the isolate formed a single distinct branch separate from those of subsp NAL101, ‘ F3-P9 and DSM 19883. The DNA–DNA hybridization values between the novel strain and the reference strains were lower than the accepted bacterial threshold level of 70 % for species delineation. The DNA G+C content of strain 2C-7 was 70.0 mol%. Based on the data, strain 2C-7 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is 2C-7 (=CFCC 11901=KCTC 39643).

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2017-07-01
2024-03-29
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