@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001944, author = "Celador-Lera, Lorena and Menéndez, Esther and Peix, Alvaro and Igual, José M. and Velázquez, Encarna and Rivas, Raúl", title = "Rhizobium zeae sp. nov., isolated from maize (Zea mays L.) roots", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "7", pages = "2306-2311", doi = "https://doi.org/10.1099/ijsem.0.001944", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001944", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "maize", keywords = "Zea mays", keywords = "Spain", keywords = "Rhizobium", keywords = "endophyte", abstract = "A bacterial strain designated CRZM18RT was isolated from a root of Zea mays in Spain. The analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Rhizobium , with Rhizobium cellulosilyticum ALA10B2T and Rhizobium yantingense H66T being the most closely related species with 98.3 and 97.9 % sequence similarity, respectively. The analysis of the concatenated recA and atpD genes showed that strain CRZM18RT forms a cluster with these species and also with Rhizobium smilacinae PTYR-5T, but the recA and atpD genes of strain CRZM18RT were phylogenetically distant, with identities lower than 90 and 96 %, respectively. DNA–DNA hybridization analysis showed mean relatedness of 43, 22 and 38 % with respect to R. cellulosilyticum ALA10B2T, R. yantingense LMG 28229T and R. smilacinae LMG 27604T. Phenotypic characteristics also differed from those of the most closely related species of the genus Rhizobium . The major fatty acids were those from summed feature 8 (C18 : 1ω6c/C18  : 1ω7c) and C16 : 0. Based on the genotypic, chemotaxonomic and phenotypic data obtained in this study, we propose to classify strain CRZM18RT in a novel species named Rhizobium zeae sp. nov. (type strain CRZM18RT=LMG 29735T=CECT 9169T).", }