1887

Abstract

Obligately anaerobic, Gram-stain-positive, spore-forming bacteria indistinguishable by pulsed-field gel electrophoresis were isolated from non-dairy protein shakes in bloated bottles. One of the isolates, strain IEH 97212, was selected for further study. The strain was closely related to and Group 1 based on 16S rRNA gene sequence similarities. Phylogenetic analysis also showed that strain IEH 97212 and strain PE (=DSM 18688), a bacterium isolated from solfataric mud, had identical 16S rRNA gene sequences. Strains IEH 97 212 and DSM 18 688 were relatively more thermophilic (temperature range for growth: 30–55 °C) and less halotolerant [growth range: 0–2.5 % (w/v) NaCl] than and They were negative for catalase, oxidase, urease and -pyrrolidonyl-arylamidase and did not produce indole. The strains produced acid from -glucose, maltose and trehalose, and hydrolysed gelatin, but did not hydrolyse aesculin. The end-products of growth included acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, isocaproic acid, phenylpropionic acid, 2-piperidinone, 2-pyrrolidinone and gas(es). The predominant fatty acids were C, C and Cω9. The genomic DNA G+C content of strains IEH 97212 and DSM 18688 was 26.9 and 26.7 mol%, respectively. According to the digital DNA–DNA hybridization data, the relatedness of these strains was 98.4 %, while they showed only 35.7–36.0 % relatedness to . Based on the results of this polyphasic study, these strains represent a novel species, for which the name sp. nov. is proposed, with the type strain IEH 97212 (=NRRL B-65463=DSM 104389).

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2017-07-01
2024-03-28
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References

  1. Collins MD, Rodrigues UM, Dainty RH, Edwards RA, Roberts TA. Taxonomic studies on a psychrophilic Clostridium from vacuum-packed beef: description of Clostridium estertheticum sp. nov. FEMS Microbiol Lett 1992; 75:235–240[PubMed]
    [Google Scholar]
  2. Broda DM, Saul DJ, Lawson PA, Bell RG, Musgrave DR. Clostridium gasigenes sp. nov., a psychrophile causing spoilage of vacuum-packed meat. Int J Syst Evol Microbiol 2000; 50:107–118 [View Article][PubMed]
    [Google Scholar]
  3. Doyle CJ, Gleeson D, Jordan K, Beresford TP, Ross RP et al. Anaerobic sporeformers and their significance with respect to milk and dairy products. Int J Food Microbiol 2015; 197:77–87 [View Article][PubMed]
    [Google Scholar]
  4. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA et al. Foodborne illness acquired in the United States-major pathogens. Emerg Infect Dis 2011; 17:7–15 [View Article][PubMed]
    [Google Scholar]
  5. Sobel J. Botulism. Clin Infect Dis 2005; 41:1167–1173 [View Article][PubMed]
    [Google Scholar]
  6. Hill KK, Smith TJ, Helma CH, Ticknor LO, Foley BT et al. Genetic diversity among botulinum neurotoxin-producing clostridial strains. J Bacteriol 2007; 189:818–832 [View Article][PubMed]
    [Google Scholar]
  7. Iyer AV, Blinkova AL, Yang SY, Harrison M, Tepp WH et al. Clostridium taeniosporum is a close relative of the Clostridium botulinum Group II. Anaerobe 2008; 14:318–324 [View Article][PubMed]
    [Google Scholar]
  8. Krasil'nikov NA, Duda VI, Pivovarov GE. Spore structure of two new species of anaerobic bacteria – Clostridium taeniosporum n. sp. and Bacillus penicillus n. sp. Microbiology 1968; 37:395–401 [Translation of the article in Russian: Mikrobiologiya 1968;37:488–493] [PubMed]
    [Google Scholar]
  9. Hill KK, Xie G, Foley BT, Smith TJ, Munk AC et al. Recombination and insertion events involving the botulinum neurotoxin complex genes in Clostridium botulinum types A, B, E and F and Clostridium butyricum type E strains. BMC Biol 2009; 7:66 [View Article][PubMed]
    [Google Scholar]
  10. Franciosa G, Ferreira JL, Hatheway CL. Detection of type A, B, and E botulism neurotoxin genes in Clostridium botulinum and other Clostridium species by PCR: evidence of unexpressed type B toxin genes in type A toxigenic organisms. J Clin Microbiol 1994; 32:1911–1917[PubMed]
    [Google Scholar]
  11. Collins MD, East AK. Phylogeny and taxonomy of the food-borne pathogen Clostridium botulinum and its neurotoxins. J Appl Microbiol 1998; 84:5–17 [View Article][PubMed]
    [Google Scholar]
  12. Smith TJ, Hill KK, Raphael BH. Historical and current perspectives on Clostridium botulinum diversity. Res Microbiol 2015; 166:290–302 [View Article][PubMed]
    [Google Scholar]
  13. Doyle ME, Glass K. Spores of Clostridium botulinum in dried dairy products. FRI Sponsors Science News Alert 2013 Aug 28
    [Google Scholar]
  14. Dobritsa AP, Reddy MC, Samadpour M. Reclassification of Herbaspirillum putei as a later heterotypic synonym of Herbaspirillum huttiense, with the description of H. huttiense subsp. huttiense subsp. nov. and H. huttiense subsp. putei subsp. nov., comb. nov., and description of Herbaspirillum aquaticum sp. nov. Int J Syst Evol Microbiol 2010; 60:1418–1426 [View Article][PubMed]
    [Google Scholar]
  15. Dobritsa AP, Kutumbaka KK, Samadpour M. Reclassification of Paraburkholderia panaciterrae (Farh et al. 2015) Dobritsa & Samadpour 2016 as a later synonym of Paraburkholderia ginsengiterrae (Farh et al. 2015) Dobritsa & Samadpour 2016. Int J Syst Evol Microbiol 2016; 66:4085–4087 [View Article][PubMed]
    [Google Scholar]
  16. Dobritsa AP, Samadpour M. Transfer of eleven species of the genus Burkholderia to the genus Paraburkholderia and proposal of Caballeronia gen. nov. to accommodate twelve species of the genera Burkholderia and Paraburkholderia . Int J Syst Evol Microbiol 2016; 66:2836–2846 [View Article][PubMed]
    [Google Scholar]
  17. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2005; 33:152–155
    [Google Scholar]
  18. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [CrossRef]
    [Google Scholar]
  19. Poehlein A, Riegel K, König SM, Leimbach A, Daniel R et al. Genome sequence of Clostridium sporogenes DSM 795T, an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum . Stand Genomic Sci 2015; 10:40 [View Article][PubMed]
    [Google Scholar]
  20. Sebaihia M, Peck MW, Minton NP, Thomson NR, Holden MT et al. Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes. Genome Res 2007; 17:1082–1092 [View Article][PubMed]
    [Google Scholar]
  21. Ionata E, Canganella F, Bianconi G, Benno Y, Sakamoto M et al. A novel keratinase from Clostridium sporogenes bv. pennavorans bv. nov., a thermotolerant organism isolated from solfataric muds. Microbiol Res 2008; 163:105–112 [View Article][PubMed]
    [Google Scholar]
  22. MacFaddin JF. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. New York: Lippincott Williams & Wilkins; 2000
    [Google Scholar]
  23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  24. Cato EP, George WL, Finegold SM. Genus Clostridium Prazmowski 1880, 23AL . In Sneath PHA, Mair NS, Sharpe ME, Holt JG. (editors) Bergey’s Manual of Systematic Bacteriology vol. 2 Baltimore, MD: Williams & Wilkins; 1986 pp. 1141–1200
    [Google Scholar]
  25. Elsden SR, Hilton MG, Waller JM. The end products of the metabolism of aromatic amino acids by clostridia. Arch Microbiol 1976; 107:283–288 [View Article][PubMed]
    [Google Scholar]
  26. Stickland LH. Studies in the metabolism of the strict anaerobes (genus Clostridium): The chemical reactions by which Cl. sporogenes obtains its energy. Biochem J 1934; 28:1746–1759[PubMed] [CrossRef]
    [Google Scholar]
  27. Nakano K, Terabayashi Y, Shiroma A, Shimoji M, Tamotsu H et al. First complete genome sequence of Clostridium sporogenes DSM 795T, a nontoxigenic surrogate for Clostridium botulinum, determined using PacBio single-molecule real-time technology. Genome Announc 2015; 3:e00832-15 [View Article][PubMed]
    [Google Scholar]
  28. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
    [Google Scholar]
  29. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article][PubMed]
    [Google Scholar]
  30. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
    [Google Scholar]
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