1887

Abstract

We herein report the isolation and characterization of 21 Gram-stain-negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of , phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (, , , and ), and whole genome sequencing and comparison between members of the genera and provided evidence for assigning them to the genus . Cellular fatty acids included important amounts of C, C, C and C. Digital DNA–DNA hybridization between the isolates Sch538 and ATCC 23330 revealed relatedness of 19.9 %. Comparative analysis of 16S rRNA gene sequences available in GenBank allowed matches to strains isolated in the USA, suggesting a wider geographical distribution. A novel species named sp. nov. is proposed, as most strains have been isolated in the Negev, a desert region of southern Israel. The type strain is Sch538 (=CCUG 69806=CSUR P957).

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2017-07-01
2024-03-28
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