1887

Abstract

A pale-red-pigmented bacterial strain, designated CW1, was isolated from a polluted soil sample in China and was characterized by a polyphasic taxonomic approach. Strain CW1 was Gram-stain-positive (or variable), coccoid, motile by a single polar flagellum and non-spore-forming. Growth was observed at 15–37 °C, but not at 10 °C or 40 °C, at pH 5.0–9.0 and with 0–5 % NaCl (w/v). Phylogenetic analysis showed that strain CW1 belongs to the genus . The 16S rRNA gene sequence similarities between CW1 and the four most closely related type strains, DSM 14505, DSM 24743, XN13 and NBRC 12536 were 97.96, 97.83, 97.83 and 97.82 %, respectively. The whole-cell sugars contained galactose, ribose and glucose. The major respiratory quinone was MK-7 followed by MK-8, and the major fatty acids were anteiso-C and anteiso-C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphoglycolipid. The DNA G+C content was 48.6 mol%. The phylogenetic and chemotaxonomic analyses indicated that strain CW1 belongs unanimously to the genus . However, DNA–DNA hybridization showed relatively low relatedness of 21.8 % (DSM 14505) and 19.6 % (DSM 24743) with the two most closely related strains of the genus . Combined with the genotypic and phenotypic analysis, strain CW1 should represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CW1 (=CCTCC AB 207187=LMG 24442).

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2017-08-01
2024-03-28
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