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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 8

sp. nov., a bacterium of the family isolated from forest soil Free

Abstract

Two strains of bacteria designated CB-3 and CB-31 were isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. They were able to grow at 15–42 °C, pH 5.5–9.5 and with 0–1.5 % (w/v) NaCl concentration. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains CB-3 and CB-31 formed a lineage within the family of the phylum that was distinct from various species of the genus , including UCM-46 (99.58 % sequence similarity), GH29-5 (98.12 %), UCM-R36 (97.57 %), UCM-R15 (96.8 %) and R2A-7 (96.31 %). Both strains contained MK-6 as the sole quinone. The major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were iso-C, iso-C 3-OH, iso-C 3-OH, summed feature 9 (iso-Cω9 and/or C 10-methyl) and iso-C G. The DNA G+C content of the strains was 35.8–36.7 mol%. DNA–DNA relatedness between strain CB-3 and the most closely related members of the genus ranged from 32 % to 59 %. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished strains CB-3 and CB-31 from their closest phylogenetic neighbours. Thus, strains CB-3 and CB-31 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CB-3 (=KEMB 9005-535=KACC 19112=NBRC 112704).

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  • Accepted:
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Keyword(s): Flavobacteriaceae , Flavobacterium flaviflagrans sp. nov. and forest soil
© 2017 IUMS
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2017-08-01
2024-04-27
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Flavobacterium flaviflagrans sp. nov., a bacterium of the family Flavobacteriaceae isolated from forest soil
Int J Syst Evol Microbiol 67, 2653 (2017); https://doi.org/10.1099/ijsem.0.002000
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