RT Journal Article SR Electronic(1) A1 Chun, Byung Hee A1 Lee, Hyo Jung A1 Jeong, Sang Eun A1 Schumann, Peter A1 Jeon, Che OkYR 2017 T1 Leucobacter ruminantium sp. nov., isolated from the bovine rumen JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 8 SP 2634 OP 2639 DO https://doi.org/10.1099/ijsem.0.002003 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, lemon yellow-pigmented, non-motile, rod-shaped bacterium, designated strain A2T, was isolated from the rumen of cow. Cells were catalase-positive and weakly oxidase-positive. Growth of strain A2T was observed at 25–45 °C (optimum, 37–40 °C), at pH 5.5–9.5 (optimum, pH 7.5) and in the presence of 0–3.5 % (w/v) NaCl (optimum, 1 %). Strain A2T contained iso-C16 : 0 and anteiso-C15 : 0 as the major cellular fatty acids. Menaquinone-11 was detected as the sole respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2T formed a distinct phyletic lineage within the genus Leucobacter . Strain A2T was most closely related to ‘ Leucobacter margaritiformis ’ A23 (97.7 % 16S rRNA gene sequence similarity) and Leucobacter tardus K 70/01T (97.2 %). The major polar lipids of strain A2T were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. Strain A2T contained a B-type cross-linked peptidoglycan based on 2,4-diaminobutyric acid as the diagnostic diamino acid with threonine, glycine, alanine and glutamic acid but lacking 4-aminobutyric acid. The G+C content of the genomic DNA was 67.0 %. From the phenotypic, chemotaxonomic and molecular features, strain A2T was considered to represent a novel species of the genus Leucobacter , for which the name Leucobacter ruminantium sp. nov. is proposed. The type strain is A2T (=KACC 17571T=JCM 19316T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002003