@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002004, author = "Huang, Jing and Li, Jiahong and Cao, Min and Liao, Shuijiao and Wang, Gejiao", title = "Cumulibacter manganitolerans gen. nov., sp. nov., isolated from sludge of a manganese mine", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "8", pages = "2646-2652", doi = "https://doi.org/10.1099/ijsem.0.002004", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002004", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Cumulibacter manganitolerans", keywords = "manganese resistance", abstract = "A Gram-stain-positive actinobacterium, designated as strain 2-36T, was isolated from sludge of a sewage outlet in a manganese mine. Phylogenetic analysis of 16S rRNA genes assigned strain 2-36T in a single lineage in the family Geodermatophilaceae and closely related to Modestobacter roseus KLBMP 1279T (93.8 % similarity), Blastococcus saxobsidens BC444T (93.1 %) and Geodermatophilus aquaeductus BMG801T (92.8 %). It contained iso-C16 : 0, iso-C15 : 0, C17 : 1ω6c, iso-C14 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) as the major fatty acids (>5 %), with MK-9(H4), MK-8(H4) and MK-9(H6) as the quinones. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the DNA G+C content was 70.1 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unknown glycolipid, an unknown aminophospholipid and two unknown phospholipids. Compared to closely related strains, strain 2-36T showed distinguishing characteristics, such as the distinct phylogenetic lineage, positive result for phenylacetic acid assimilation and negative result for α-glucosidase and having C17 : 1ω6c and iso-C14 : 0 as the major fatty acids. On the basis of the polyphasic analyses, strain 2-36T represents a novel species of a new genus within the family Geodermatophilaceae, for which the name Cumulibacter manganitolerans gen. nov., sp. nov. is proposed. The type strain of 'Cumulibacter manganitolerans' is 2-36T (=CCTCC AA 2016026T=DSM 103787T).", }