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Volume 67, Issue 8

gen. nov. sp. nov., isolated from sediment Free

Abstract

A Gram-stain-positive, aerobic, motile, endospore-forming bacterium, designated strain J15A17, was isolated from sediment of the South China Sea. The strain was oxidase-positive and catalase-negative. Optimal growth occurred at 33 °C, pH 7.5 and in the presence of 3 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, the strain showed closest similarity (92.8 %) to strain CAU 9324. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family , with the genus as the most closely related genus. The DNA G+C content of strain J15A17 was 37.4 mol%. The strain contained MK-7 as the sole respiratory quinone; anteiso-C and iso-C were the major cellular fatty acids; and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and four unidentified phospholipids. The strain displayed the peptidoglycan type A4α -Lys–-Asp in the cell wall. Phylogenetic, physiological, biochemical and morphological differences between strain J15A17 and its closest relatives in the genera , and suggest that strain J15A17 (=KCTC 33759=MCCC 1H00137) represents the type strain of a novel species in a new genus within the family , gen. nov. sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Chengkuizengella sediminis gen. nov. sp. nov. , Marine bacteria , sediment and South China Sea
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2017-08-01
2024-04-19
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Chengkuizengella sediminis gen. nov. sp. nov., isolated from sediment
Int J Syst Evol Microbiol 67, 2672 (2017); https://doi.org/10.1099/ijsem.0.002006
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