@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002014, author = "Ojha, Anup Kumar and Verma, Ashish and Pal, Yash and Bhatt, Deepak and Mayilraj, Shanmugam and Krishnamurthi, Srinivasan", title = "Marinomonas epiphytica sp. nov., isolated from a marine intertidal macroalga", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "8", pages = "2746-2751", doi = "https://doi.org/10.1099/ijsem.0.002014", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002014", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "phylogeny", keywords = "SAB-3T", keywords = "16S rRNA", keywords = "FAME", keywords = "MALDI-TOF", abstract = "A novel Gram-stain-negative, aerobic marine bacterial strain, SAB-3T, was isolated from brown macroalgae (Dictyota sp.) growing in the Arabian sea, Goa, India. The strain grew optimally at 30 °C, with 2.0–4.0 % (w/v) NaCl and at pH 7.0 on marine agar medium. Strain SAB-3T was unable to hydrolyse aesculin and did not grow in the presence of rifamycin but showed resistance to antibiotics such as cefadroxil and co-trimoxazole. The major fatty acids were summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c), summed feature 3 (C16 : 1 ω7c/C16 : 1 ω 6c) and C16 : 0, and Q-8 was the major ubiquinone. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 41.0 mol%. 16S rRNA gene sequencing and phylogenetic analysis indicated that the strain was a member of the genus Marinomonas with Marinomonas aquiplantarum IVIA-Po-159T (97.6 % similarity), Marinomonas posidonica IVIA-Po-181T (97.5 %) and Marinomonas dokdonensis DSM 17202T (97.4 %) as the closest relatives. Whole genome relatedness determined through DNA–DNA hybridization revealed values of 40–50 % (below the 70 % threshold recommended for species delineation) with the above three species, thus confirming it as representing a distinct and novel species of the genus Marinomonas for which the name Marinomonas epiphytica sp. nov. is proposed. The type strain is SAB-3T (=JCM 31365T=KCTC 52293T=MTCC 12569T).", }