%0 Journal Article %A Sánchez-Castro, Iván %A Ruiz-Fresneda, Miguel Angel %A Bakkali, Mohammed %A Kämpfer, Peter %A Glaeser, Stefanie P. %A Busse, Hans Jürgen %A López-Fernández, Margarita %A Martínez-Rodríguez, Pablo %A Merroun, Mohamed Larbi %T Stenotrophomonas bentonitica sp. nov., isolated from bentonite formations %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 8 %P 2779-2786 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002016 %K bentonite %K ANI value %K Stenotrophomonas %K BII-R7T %I Microbiology Society, %X A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7T represented a member of the genus Stenotrophomonas (class Gammaproteobacteria ), and was related most closely to Stenotrophomonas rhizophila e-p10T (99.2 % sequence similarity), followed by Stenotrophomonas pavanii ICB 89T (98.5 %), Stenotrophomonas maltophilia IAM 12423T, Stenotrophomonas chelatiphaga LPM-5T and Stenotrophomonas tumulicola T5916-2-1bT (all 98.3 %). Pairwise sequence similarities to all other type strains of species of the genus Stenotrophomonas were below 98 %. Genome-based calculations (orthologous average nucleotide identity, original average nucleotide identity, genome-to-genome distance and DNA G+C percentage) indicated clearly that the isolate represents a novel species within this genus. Different phenotypic analyses, such as the detection of a quinone system composed of the major compound ubiquinone Q-8 and a fatty acid profile with iso-C15 : 0 and anteiso-C15 : 0 as major components, supported this finding at the same time as contributing to a comprehensive characterization of BII-R7T. Based on this polyphasic approach comprising phenotypic and genotypic/molecular characterization, BII-R7T can be differentiated clearly from its phylogenetic neighbours, establishing a novel species for which the name Stenotrophomonas bentonitica sp. nov. is proposed with BII-R7T as the type strain (=LMG 29893T=CECT 9180T=DSM 103927T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002016