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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 8

sp. nov., isolated from saline–alkaline soil Free

Abstract

A Gram-staining-negative, non-motile, aerobic bacterial strain, designated Y3L17, was isolated from the saline–alkaline soil of a farmland, Hangjin Banner, Inner Mongolia, northern China. Y3L17 could grow at 15–45 °C (optimum 35 °C), pH 6.0–10.0 (optimum pH 8.0) and with 0–4 % (w/v) NaCl (optimum 0 %). The results of phylogenetic analysis based on the 16S rRNA gene and gene sequences revealed that Y3L17 tightly clustered with strains of members of the genus , sharing the highest 16S rRNA gene similarities with S2-21 (99.5 %) and HO3-R19 (98.2 %), and lower similarities (<97 %) with all the other type strains of species of this genus. However, Y3L17 shared only 92.62 % gene similarities with S2-21. The DNA–DNA hybridization values of Y3L17 with S2-21 and HO3-R19 were 20.1±2.5 and 18.2±3.2 %, respectively. Y3L17 contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, five unknown phospholipids and one unknown lipid as the major polar lipids. Ubiquinone-8 (Q-8) was the predominant respiratory quinone, while iso-C, iso-Cω9 and iso-C 3-OH were the major cellular fatty acids. Its genomic DNA GC content was 65.4 mol%. On the basis of its phenotypic, phylogenetic and genotypic characteristics, Y3L17 represents a novel species within the genus , for which the name sp. nov. is proposed, the type strain is Y3L17 (=CGMCC 1.15905 =KCTC 52420).

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Keyword(s): Arenimonas soli sp. nov. and saline-alkaline soil
© 2017 IUMS
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2017-08-01
2024-04-24
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References

  1. Kwon SW, Kim BY, Weon HY, Baek YK, Go SJ. Arenimonas donghaensis gen. nov., sp. nov., isolated from seashore sand. Int J Syst Evol Microbiol 2007; 57:954–958 [View Article][PubMed]
     [Google Scholar]
  2. Aslam Z, Park JH, Kim SW, Jeon CO, Chung YR. Arenimonas oryziterrae sp. nov., isolated from a field of rice (Oryza sativa L.) managed under a no-tillage regime, and reclassification of Aspromonas composti as Arenimonas composti comb. nov. Int J Syst Evol Microbiol 2009; 59:2967–2972 [View Article][PubMed]
     [Google Scholar]
  3. Zhang SY, Xiao W, Xia YS, Wang YX, Cui XL et al. Arenimonas taoyuanensis sp. nov., a novel bacterium isolated from rice-field soil in China. Antonie van Leeuwenhoek 2015; 107:1181–1187 [View Article][PubMed]
     [Google Scholar]
  4. Jin L, Kim KK, Im WT, Yang HC, Lee ST. Aspromonas composti gen. nov., sp. nov., a novel member of the family Xanthomonadaceae. Int J Syst Evol Microbiol 2007; 57:1876–1880 [View Article][PubMed]
     [Google Scholar]
  5. Huy H, Jin L, Lee YK, Lee KC, Lee JS et al. Arenimonas daechungensis sp. nov., isolated from the sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 2013; 63:484–489 [View Article][PubMed]
     [Google Scholar]
  6. Kim AR, Lee S, Han K, Ahn TY. Arenimonas aquatica [corrected] sp. nov., a member of the gammaproteobacterium, isolated from a freshwater reservoir. J Microbiol 2012; 50:354–358 [View Article][PubMed]
     [Google Scholar]
  7. Yuan X, Nogi Y, Tan X, Zhang RG, Lv J. Arenimonas maotaiensis sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 2014; 64:3994–4000 [View Article][PubMed]
     [Google Scholar]
  8. Young CC, Kämpfer P, Ho MJ, Busse HJ, Huber BE et al. Arenimonas malthae sp. nov., a gammaproteobacterium isolated from an oil-contaminated site. Int J Syst Evol Microbiol 2007; 57:2790–2793 [View Article][PubMed]
     [Google Scholar]
  9. Chen F, Shi Z, Wang G. Arenimonas metalli sp. nov., isolated from an iron mine. Int J Syst Evol Microbiol 2012; 62:1744–1749 [View Article][PubMed]
     [Google Scholar]
  10. Jin L, Kim KK, An KG, Oh HM, Lee ST. Arenimonas daejeonensis sp. nov., isolated from compost. Int J Syst Evol Microbiol 2012; 62:1674–1678 [View Article][PubMed]
     [Google Scholar]
  11. Jeong HI, Jin HM, Jeon CO. Arenimonas aestuarii sp. nov., isolated from estuary sediment. Int J Syst Evol Microbiol 2016; 66:1527–1532 [View Article][PubMed]
     [Google Scholar]
  12. Sun JQ, Liu M, Wang XY, Xu L, Wu XL. Sphingobacterium suaedae sp. nov., isolated from the rhizosphere soil of Suaeda corniculata. Int J Syst Evol Microbiol 2015; 65:4508–4513 [View Article][PubMed]
     [Google Scholar]
  13. Sun JQ, Xu L, Liu M, Wang XY, Wu XL. Flavobacterium suaedae sp. nov., an endophyte isolated from the root of Suaeda corniculata. Int J Syst Evol Microbiol 2016; 66:1943–1949 [View Article][PubMed]
     [Google Scholar]
  14. Sun JQ, Xu L, Wu XL. Lysinibacillus alkalisoli sp. nov., isolated from saline–alkaline soil. Int J Syst Evol Microbiol 2017; 67:67–71 [View Article][PubMed]
     [Google Scholar]
  15. Xu L, Sun JQ, Wang LJ, Liu XZ, Ji YY et al. Aliidiomarina soli sp. nov., isolated from saline-alkaline soil. Int J Syst Evol Microbiol 2017; 67:724–728 [View Article][PubMed]
     [Google Scholar]
  16. Xu L, Sun J-Q, Wang L-J, Gao Z-W, Sun L-Z et al. Sphingobacterium alkalisoli sp. nov., isolated from saline–alkaline soil. Int J Syst Evol Microbiol 2017
     [Google Scholar]
  17. Wang YN, Chi CQ, Cai M, Lou ZY, Tang YQ et al. Amycolicicoccus subflavus gen. nov., sp. nov., an actinomycete isolated from a saline soil contaminated by crude oil. Int J Syst Evol Microbiol 2010; 60:638–643 [View Article][PubMed]
     [Google Scholar]
  18. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  20. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  21. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  22. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1992; 9:945–967
     [Google Scholar]
  23. Rzhetsky A, Nei M. Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 1993; 10:1073–1095[PubMed]
     [Google Scholar]
  24. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  25. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  26. Mandel M, Marmur J. Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 1968; 12B:195 [CrossRef]
     [Google Scholar]
  27. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [View Article][PubMed]
     [Google Scholar]
  28. Huß VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4:184–192 [View Article][PubMed]
     [Google Scholar]
  29. Makk J, Homonnay ZG, Kéki Z, Nemes-Barnás K, Márialigeti K et al. Arenimonas subflava sp. nov., isolated from a drinking water network, and emended description of the genus Arenimonas. Int J Syst Evol Microbiol 2015; 65:1915–1921 [View Article][PubMed]
     [Google Scholar]
  30. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
     [Google Scholar]
  31. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [CrossRef]
     [Google Scholar]
  32. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  33. Kates M. Techniques of Lipidology, 2nd ed. Amsterdam: Elsevier; 1986
     [Google Scholar]
  34. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
     [Google Scholar]
  35. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  36. Dong XZ, Cai MY. Determinative Manual for Routine Bacteriology Beijing, China: Scientific Press; 2001
     [Google Scholar]
  37. Kim BC, Jeong WJ, Kim DY, Oh HW, Kim H et al. Paenibacillus pueri sp. nov., isolated from Pu'er tea. Int J Syst Evol Microbiol 2009; 59:1002–1006 [View Article][PubMed]
     [Google Scholar]
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Arenimonas soli sp. nov., isolated from saline–alkaline soil
Int J Syst Evol Microbiol 67, 2829 (2017); https://doi.org/10.1099/ijsem.0.002026
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