%0 Journal Article %A Khan, Inam Ullah %A Hussain, Firasat %A Habib, Neeli %A Xiao, Min %A Ahmed, Iftikhar %A Amin, Arshia %A Zhi, Xiao-Yang %A Li, Wen-Jun %T Nocardioides thalensis sp. nov., isolated from a desert %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 8 %P 2848-2852 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002032 %K Pakistan %K Nocardioides thalensis sp. nov. %K Thal desert %K polyphasic taxonomy approach %I Microbiology Society, %X A novel actinobacterial strain, designated NCCP-696T, was isolated from the Thal desert in Punjab, Pakistan, and characterized by using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NCCP-696T belongs to the genus Nocardioides and showed the highest level of sequence similarity with respect to Nocardioides panacisoli Gsoil 346T (98.2 %) and less than 96.4 % to the strains of other species of the genus Nocardioides . Cells of strain NCCP-696T were Gram-positive, aerobic, non-motile rods and formed cream-coloured colonies. The strain was positive for oxidase and catalase. Growth occurred at 20–42 °C (optimum 30–37 °C) at pH 5.5–9.0 (optimum pH 7.0) and with 0–4 % NaCl (optimum 0–2 %, w/v). Strain NCCP-696T contained Iso-C16 : 0, C18 : 1ω9c, C17 : 1ω8c and C17 : 0 as the predominant fatty acids and was found to have LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan. The polar lipid profile consisted of phosphatidylinositol, phosphatidylglycerol and one unknown phospholipid. The major menaquinone was MK-8(H4) (98.7 %) while a minor amount (1.3 %) of MK-9(H2) was also detected. The DNA G+C content of the genomic DNA was 71.6 mol%. The DNA–DNA hybridization value of the isolate against the closely related type strain Nocardioides panacisoli Gsoil 346T was 56.3±1.4. On the basis of the phylogenetic inference, chemotaxonomic characteristics and phenotypic data, strain NCCP-696T should be classified as a novel species, for which the name Nocardioides thalensis sp. nov. is proposed. The type strain is NCCP-696T (=DSM 103833T=CCTCC AB 2016296T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002032