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Volume 67, Issue 8

sp. nov., isolated from soil of a military shooting range Free

Abstract

A Gram-stain-negative, strictly aerobic bacterium, designated R1-4, was isolated from soil from a military shooting range in the Republic of Korea. Cells were non-motile short rods, oxidase-positive and catalase-negative. Growth of R1-4 was observed at 15–45 °C (optimum, 30 °C) and pH 6.0–9.0 (optimum, pH 7.0). R1-4 contained summed feature 8 (comprising Cω7/C ω6), summed feature 3 (comprising Cω7/C ω6), cyclo-Cω8 and C as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, an unknown glycolipid and four unknown lipids were detected as polar lipids. The major polyamine was spermidine. The G+C content of the genomic DNA was 64.4 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that R1-4 formed a tight phylogenetic lineage with HU1-AH51 within the genus . R1-4 was most closely related to HU1-AH51 with a 98.8 % 16S rRNA gene sequence similarity. The DNA–DNA relatedness between R1-4 and the type strain of was 37.8±4.2 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that R1-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R1-4 (=KACC 19094=JCM 31879).

  • Received:
  • Accepted:
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Keyword(s): new taxa , Novosphingobium humi , shooting range and soil
© 2017 IUMS
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2017-08-01
2024-04-16
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Novosphingobium humi sp. nov., isolated from soil of a military shooting range
Int J Syst Evol Microbiol 67, 3083 (2017); https://doi.org/10.1099/ijsem.0.002089
/content/journal/ijsem/10.1099/ijsem.0.002089
/content/journal/ijsem/10.1099/ijsem.0.002089
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