%0 Journal Article %A Naghoni, Ali %A Emtiazi, Giti %A Amoozegar, Mohammad Ali %A Rasooli, Mehrnoosh %A Etemadifar, Zahra %A Shahzadeh Fazeli, Seyed Abolhassan %A Minegishi, Hiroaki %A Ventosa, Antonio %T Natronoarchaeum persicum sp. nov., a haloarchaeon isolated from a hypersaline lake %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 9 %P 3339-3344 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002116 %K Natronoarchaeum persicum %K Lake Meyghan %K 16S rRNA %I Microbiology Society, %X A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypersaline lake in Iran. Cells of strain WIIAL99T were non-motile, catalase-positive and oxidase-negative. Strain WIIAL99T required at least 2.5 M NaCl and 0.05 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 37–40 °C; it was able to grow at pH 6.0–8.5 and 20–55 °C. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain WIIAL99T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether and one unidentified glycolipid. The DNA G+C content of strain WIIAL99T was 66.7 mol%. The closest relative was Natronoarchaeum rubrum JCM 17119T with 98.2 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB′ gene sequences indicated that strain WIIAL99T is a member of the genus Natronoarchaeum in the family Halobacteriaceae and forms a distinct cluster. It was concluded that strain WIIAL99T (=IBRC-M 11062T=LMG 29814T) represents a novel species of the genus Natronoarchaeum , for which the name Natronoarchaeum persicum sp. nov. is proposed. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002116