RT Journal Article SR Electronic(1) A1 Naghoni, Ali A1 Emtiazi, Giti A1 Amoozegar, Mohammad Ali A1 Rasooli, Mehrnoosh A1 Etemadifar, Zahra A1 Shahzadeh Fazeli, Seyed Abolhassan A1 Minegishi, Hiroaki A1 Ventosa, AntonioYR 2017 T1 Natronoarchaeum persicum sp. nov., a haloarchaeon isolated from a hypersaline lake JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 9 SP 3339 OP 3344 DO https://doi.org/10.1099/ijsem.0.002116 PB Microbiology Society, SN 1466-5034, AB A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypersaline lake in Iran. Cells of strain WIIAL99T were non-motile, catalase-positive and oxidase-negative. Strain WIIAL99T required at least 2.5 M NaCl and 0.05 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 37–40 °C; it was able to grow at pH 6.0–8.5 and 20–55 °C. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain WIIAL99T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether and one unidentified glycolipid. The DNA G+C content of strain WIIAL99T was 66.7 mol%. The closest relative was Natronoarchaeum rubrum JCM 17119T with 98.2 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB′ gene sequences indicated that strain WIIAL99T is a member of the genus Natronoarchaeum in the family Halobacteriaceae and forms a distinct cluster. It was concluded that strain WIIAL99T (=IBRC-M 11062T=LMG 29814T) represents a novel species of the genus Natronoarchaeum , for which the name Natronoarchaeum persicum sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002116