RT Journal Article SR Electronic(1) A1 Hamada, Moriyuki A1 Saitou, Satomi A1 Terahara, Takeshi A1 Imada, Chiaki A1 Tamura, TomohikoYR 2017 T1 Lysinimicrobium sediminis sp. nov., an actinobacterium isolated from estuary sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 9 SP 3393 OP 3397 DO https://doi.org/10.1099/ijsem.0.002123 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-positive actinobacterium, designated HT7-17T, was isolated from a sediment sample collected from the estuary of the Tama River, Japan, and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain HT7-17T was closely related to members of the genus Lysinimicrobium , with a similarity range of 97.1–98.2 %. The peptidoglycan type of strain HT7-17T was A4α, the predominant menaquinone was demethylmenaquinone DMK-9(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The DNA G+C content was 69.9 mol%. These chemotaxonomic features corresponded to those of the genus Lysinimicrobium . Meanwhile, the differences in some phenotypic characteristics, along with the result of DNA–DNA hybridization, indicated that strain HT7-17T should be distinguished from the recognized species of the genus Lysinimicrobium . Therefore, strain HT7-17T represents a novel species of the genus Lysinimicrobium , for which the name Lysinimicrobium sediminis sp. nov. is proposed. The type strain is HT7-17T (=NBRC 112286T=TBRC 7037T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002123