RT Journal Article SR Electronic(1) A1 Dastgheib, Seyed Mohammad Mehdi A1 Tirandaz, Hassan A1 Moshtaghi Nikou, Mahdi A1 Ramezani, Mohaddaseh A1 Shavandi, Mahmoud A1 Amoozegar, Mohammad Ali A1 Ventosa, AntonioYR 2017 T1 Prauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 9 SP 3381 OP 3386 DO https://doi.org/10.1099/ijsem.0.002124 PB Microbiology Society, SN 1466-5034, AB A crude-oil-degrading, Gram-stain-positive actinobacterial strain, RIPIT, was isolated from a soil sample collected from an oil-contaminated mud pit in Khangiran oil and gas field, in the north-east of Iran. RIPIT was strictly aerobic, catalase- and oxidase-positive. The strain grew with 0–12.5 % (w/v) NaCl (optimum 3–5 %), at 25–55 °C (optimum 45 °C) and at pH 6.0–9.5 (optimum pH 7.0). The results of 16S rRNA gene sequence comparative analysis indicated that RIPIT represents a member of the genus Prauserella , with high phylogenetic similarity to Prauserella coralliicola SCSIO 11529T (97.5 %), Prauserella endophytica SP28S-3T (97.5 %) and Prauserella marina MS498T (97.2 %). DNA–DNA relatedness values between the novel strain and P. coralliicola DSM 45821T, P. endophytica DSM 46655T and P. marina DSM 45268T were 28 , 19 and 23 %, respectively. The cell wall peptidoglycan of RIPIT contained meso-diaminopimelic acid as the diamino acid and the whole-cell sugars are galactose and arabinose. The polar lipids pattern contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. Its cellular fatty acids pattern consisted of C17 : 1ω6c, iso-C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), and the major respiratory quinone was MK-9(H4). The G+C content of the genomic DNA was 69 mol%. On the basis of polyphasic taxonomic data we propose that RIPIT represents a novel species of the genus Prauserella , for which the name Prauserella oleivorans sp. nov. is proposed. The type strain of Prauserella oleivorans is RIPIT (=IBRC-M 10906T=LMG 28389T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002124