@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002130, author = "Subhash, Y. and Lee, Sang-Seob", title = "Description of Oceanispirochaeta sediminicola gen. nov., sp. nov., an obligately anaerobic bacterium isolated from coastal marine sediments, and reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "9", pages = "3403-3409", doi = "https://doi.org/10.1099/ijsem.0.002130", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002130", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "novel genus", keywords = "Spirochaetaceae", keywords = "Oceanispirochaeta sediminicola", keywords = "coastal marine sediments", abstract = "An obligately anaerobic spirochaete (strain SY2T) was isolated from coastal marine sediments of Tongyeong-Si, South Korea. Strain SY2T was helical-shaped and Gram-stain-negative. Strain SY2T was able to grow at 10–40 °C (optima, 25–30 °C), pH 6.3–8.8 (optima, pH 7.0–8.0) and with 1–7 % (optimum, 2–3 %) NaCl concentration. Strain SY2T was negative for catalase and oxidase activity. The major end-products of glucose fermentation were acetate, ethanol, hydrogen and carbon dioxide. C14 : 0, C16 : 0, iso-C15 : 0, iso-C14 : 0 3-OH, iso-C15 : 1 H/C13 : 0 3-OH and iso-C17 : 1ω9c were predominant fatty acids (>5 %) with minor amounts (<5 %) of C18 : 0, iso-C13 : 0, iso-C17 : 0, iso-C17 : 1/anteiso-C17 : 1 B and C16 : 1ω6c/C16  : 1ω7c. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic DNA G+C content was 53.5 mol%. 16S rRNA gene sequence comparisons indicated that strain SY2T represents a member of the family Spirochaetaceae in the phylum Spirochaetes . Strain SY2T has a sequence similarity of 95.1 % with Spirochaeta litoralis R1T and <90.1 % with other members of the genus Spirochaeta . Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain SY2T as a representative of a novel genus and species in the family Spirochaetaceae , for which the name Oceanispirochaeta sediminicola gen. nov., sp. nov. is proposed. The type strain is SY2T (=KEMB 3001-381T=DSM 104770T=KCTC 15593T). Reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov. is also proposed based on polyphasic taxonomic analyses.", }