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Volume 67, Issue 9

sp. nov., isolated from a spoiled brewery sample Free

Abstract

A Gram-stain-positive, non-motile, rod-shaped bacterium, designated TUM BP 140423000-2250 (=DSM 100836=LMG 29073), was isolated from spoiled beer. This bacterium did not form spores, and was catalase-negative and facultatively anaerobic. Its taxonomic position was determined in a polyphasic study. The 16S rRNA gene sequence similarity data showed that the strain belonged to the genus with the nearest neighbours being DCY50 (sequence similarity 99.5 %), THK-V8 (99.2 %) and LMG 11984 (98.7 %). Sequence comparisons of additional phylogenetic markers, and , confirmed the 16S rRNA gene sequence tree topology. The maximum sequence similarity was 92.3 % with THK-V8. The DNA G+C content of the isolate was 50.0 mol%. The DNA–DNA relatedness showed that strain TUM BP 140423000-2250 could be clearly distinguished from DCY 50 (30.8±0.4 %) and THK-V8 (23.6±5.9 %). The major fatty acids were Cω9, summed feature 7 (comprised of C cyclo ω10/Cω6) and C. Based on phenotypic and genotypic studies, the authors propose classifying the new isolate as a representative of a novel species of the genus , sp. nov. The type strain is deposited at the Research Centre Weihenstephan for Brewing and Food Quality as TUM BP 140423000-2250 (=DSM 100836=LMG 29073).

  • Received:
  • Accepted:
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Keyword(s): beer-spoilage bacteria , Lactobacillus cerevisiae , Lactobacillus koreensis , Lactobacillus parabrevis , Lactobacillus yonginensis and spoilage
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2017-09-01
2024-04-20
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Lactobacillus cerevisiae sp. nov., isolated from a spoiled brewery sample
Int J Syst Evol Microbiol 67, 3452 (2017); https://doi.org/10.1099/ijsem.0.002139
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