%0 Journal Article %A Wang, Xiang %A Jiang, Wan-Kui %A Cui, Meng-Di %A Yang, Zhan-Gong %A Yu, Xing %A Hu, Gang %A Zhang, Hao %A Hong, Qing %T Nocardioides agrisoli sp. nov., isolated from farmland soil %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 10 %P 3722-3727 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002158 %K Nocardioides agrisoli sp. nov. %K Gram-staining-positive %K farmland soil %I Microbiology Society, %X A novel Gram-stain-positive bacterium, designated djl-8T, was isolated from farmland soil in Nanjing, Jiangsu province, PR China. Cells of strain djl-8T were aerobic, non-motile, non-spore-forming and rod-shaped. The organism grew at 25–37 °C, pH 5.5–8.0 and 0.5–4.0 % NaCl (w/v). The DNA G+C content was 69.3 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was LL-2, 6-diaminopimelic acid. The major fatty acids (>5 %) were iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, 10-Me C17 : 0 and C17 : 1ω8c. The respiratory quinone was MK-8 (H4) and the major polar lipids were phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol and unknown phospholipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain djl-8T is a member of the genus Nocardioides and shared the highest similarity with Nocardioides ginkgobilobae SYP-A7303T (97.1 %), followed by Nocardioides soli mbc-2T (96.9 %), Nocardioide spyridinolyticus OS4T (96.6 %) and Nocardioides maradonensis RP-B30T (96.6 %). Strain djl-8T exhibited low DNA–DNA relatedness with Nocardioides ginkgobilobae SYP-A7303T (26.9±2.1 %). On the basis of the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain djl-8T represents a novel species of the genus Nocardioides , for which the name Nocardioides agrisoli sp. nov. is proposed. The type strain is djl-8T (=KCTC 39844T=CCTCC AB 2017058T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002158